Study On Control Cucumber Mosaic Virus Of Tobacco Through DsRNA

In this study, in order to effectively transcribe dsRNA in plants, an inducer ofRNA silencing, CMV (Cucumber mosaic virus) partial movement protein gene(â–³MP) were in inverted repeat manner, the recombinant was then inserted intobinary vector pBIN438 under control of the 35S promoter. 34r transgenic tobaccoplants expressing CMVâ–³MP gene were generated by Agrobacteriumtumefaciens-mediated transformation, resistance and possible mechanism forresistance of these transgenic plants were evaluated.pBIN-CMV MP(i/r) was transformed into A. tumefaciens EHA 105 by tri-parentalmating method. The positive clones confirmed by PCR and digestion were used fortransformation of tobacco plants. The vector pBIN-CMV MP (i/r) was transformedinto tobacco by A. tumefaciens-mediated method via leaf disc, 30 kanamycin-resistanttransgenic plants expressing TMV MP (i/r) were obtained. PCR and Southern blotanalysis of transgenic plants showed that CMV MP (i/r) had been integrated intotobacco genome. 30 transgenic plants expressing CMV MP (i/r) were inoculated withCMV, symptom observation and ELISA detection showed that transgenic plants haveresistant to CMVOur results lead a way for plant virus control using dsRNA through PTGSapproach.