The Preparation Of "Qin Hu Kang" And Its PHarmacodynamic Studies In Chickens Induced With Mycoplasma Gallisepticum And Escherichia Coli

Objective: To study the preparation of effervescent granule of compound difloxacin hydrochloride (QIN HU KANG) and its pHarmacodynaraic against Mycoplasma gallisepticum(MG) and septico-Escherichia coli (E. coli). To offer theory guidance for the clinical application of "QIN HU KANG". Method: 1.To screen the formulation and dosage of "QIN HU KANG" by single factor method. 2. To optimize the formulation of "QIN HU KANG" by the orthogonal experiment. 3. To prepare the "QIN HU KANG" by wet granulating with non reactive liquids method. 4. Minimal inhibitory concentrations (MICs) and minimal bactericidal concentration (MBCs) of "QIN HU KANG" against MG and E. coli were determined by two-fold diltution method in vitro. 5. 270 chickens (3 weeks old) were randomly divided into 9 groups, one of 9 groups was used as uninfected and untreated group. Each chicken in six groups was first infected intratracheally (2. Oml/kg b. w) with MG broth culture prepared in FM-4 medium, containing approximately 1.1×10~8ccu/mL. Three days later, the chickens were infected again with E.coli containing 8×10~8cfu/mL into breast muscle by intramusclular injection (2.OmL/kg b. w). One of remaining two groups was only infected with MG, other only infected only E.coli. Three groups of infected chickens were treated with compound preparation orally at the dosage of 12, 10, 8mg/kg b.w, two groups infected chickens were treated with difloxacin hydrochloride and enrofloxacin hydrochloride as the positive control at a dosage of 10.0mg/kg b. w following oral administration, respectively, twice daily for 3 consecutive days. The last group infected both MG and E. coli, the group infected only MG and the group infected only E. coli were untreated as the negative control. To observe clinical signs, necropsy, antibody examination and isolation of the pathogen. Results: 1.The formulation of "QIN HU KANG" is citric acid and sodium bicarbonate as gas-producing disintegrant (1:1.5), amidulin and cane-sugar as loading agent(3:2), and fumaric-acid as lubricant. 3.The results of the optimized orthogonal experiment were the dosage of lubricant(3. 5%), the dosage of gas-producing disintegrant (62. 87g) and the dosage of disintegrant(1. 5%). 3. The artificially induced animal model is succeeded by clinical signs, necropsy, antibody examination and isolation of the pathogens. 4. The curative rates of "QIN HU KANG", difloxacin hydrochloride and enrofloxacin hydrochloride in orally treated groups were 93. 3%, 100%, 100%, 100%, 100%, respectively, but the mortality of the group infected both MG and E.coli, the group infected only MG and the group infected only E.coli were 43.3%, 0%, 33.3%, respectively. The each curative group was superior to the infected group significantly in controlling infection, increasing curing rate, effective rate and weight gain, lowing mortality, positive antibody rate, air-sac damage rate and frequency of pathogenic microorganism isolation. Conclusions: 1. To prepare the "QIN HU KANG" by 5% polyvidone anhydro-alcosol granulating with non reactive liquids method. 2. The formulation of "QIN HU KANG" is difloxacin hydrochloride(10. OOg), saponin of platycond root (40. OOg), loading agent (100. OOg), lubricant (7. 84g), gas-producing disintegrant (62. 87g), disintergrant(3. 36g). 3. The antimicrobial activity of "QIN HU KANG" is strong in vitro and (MICs) of "QIN HU KANG" against MG and E. coli were 0.2146 and 0.0816 mg/L, respectively. 4. The curative effect of the "QIN HU KANG" is significant against infected with MG and E. coli in chickens, and its optimal dosage is 10mg/kg b. w, twice daily for 3 consecutive days.