Studies On The Dietary Riboflavin, Biotin And Pantothenic Acid Requirement Of Grass Carp(Ctenopharyngodon Idellus) Fingerling

The research in this paper were done to investigate the effect of different riboflavin,biotin and pantothenic acid on the growth, feed coefficient, body compositions andbiochemical indexes of blood serum of grass carp fingerlings. The results obtained are asfollow:1. The experiment was designed to investigate the riboflavin requirement of grasscarp fingerling. Seven semi-purified diet contained different riboflavin level (0, 2, 4, 6, 10,20, 40mg/kg). The experiment was conducted in the fiberglas pool and lasted 10 week.The initial weight of fish was (22.13±1.06)g and the mean temperature of water was 25℃during the experiment. The result of the experiment showed that the supplementarydietary riboflavin could improve weight gain, specific growth rate, feed efficiency andactive of glutathione reductase activity in serum of grass carp fingerling. The highestriboflavin in liver were observed when the fish fed above 8.6mg/kg riboflavin. Carcasemoisture, ash and crude fat content of the fish were not significant affected by thesupplemental levels of dietary riboflavin. Based on the specific growth rate of grass carpfingerling, it is proposed that the optimal riboflavin requirement for grass carpfingerling growth is about 7.9mg/kg diet.2. A growth study was conducted to determine the dietary biotin requirement ofgrass carp fingerling, Purified diets with 7 levels 0, 0.05, 0.1, 0.2, 0.4, 0.8, 1.6 mg/kg ofsupplemental biotin were fed to grass carp (mean weight 5.92±0.25g) fingerling for 8weeks. Each diet was fed to three replicate groups of fish. Results indicated that highestweight gain observerd in the group fed the diet supplemented with 0.4 mg/kg, followedby fish fed the diets with 1.6, 0.8, 0.05, 0.2, 0.1 and 0mg/kg, and lowest in fish fed theunsupplemented control diet. Moisture, lipid and protein contents of body were notmarkedly affected by the supplemental levels of dietary biotin. The glucose, totalcholesterol, triacylglycerol, total protein, low density lipoprotein cholesterol and highdensity lipoprotein in grass carp fingerling serum increased with dietary biotinsupplementation. Broken-line analysis of specific growth rate on levels of dietary biotin indicated that the optimal biotin requirement for grass carp fingerling growth is about0.31mg/kg diet.3. A 8 weeks growth trial was conducted to investigate the effect of dietary ofpantothenic acid requirement for grass carp fingerling. Purified basal diets wereformulated using the casein as the protein source. Pantothenic acid was supplemented at 0,8, 15, 30, 60, 120, and 240 mg/kg diet to the basal diet. Each diet was fed to threereplicate groups of grass carp initially averaging (4.80±0.32)g. Results indicated that thesupplementary dietary pantothenic acid could improve weight gainand specific growthrate, and reduce feed conversion ratio (P＜0.05), but not affect the survival rate (P＞0.05).The total cholesterol, high density lipoprotein cholesterol and low density lipoproteincholesterol in serum of grass carp fingering in control group was significantly lower thanthose in experimental groups fed pantothenic acid enriched diet(P＜0.05). The carcaseprotein and lipid content increased significantly by the supplemental levels of dietarypantothenic acid, whereas the ash content were not affected. Based on the specific growthrate of grass carp fingerling, it is proposed that the optimal pantothenic acid for grass carpfingerling growth is about 25mg/kg diet.