Preparation And Rudiment Study On Pharmacy And Stability Of Ceftiofur Sodium Liposome

Liposome is a new drug delivery. It has been widely used in medical area. Liposome has many characters: longest effect, highest effect, lowest toxity, lowest side effect, Ceftiofur is one of new pharmaceutical on the market. It is used treating animal disease. Our objection is to study the stable preparation of ceftiofur sodium-liposome. In the paperwe also study the pharmacy and quality evaluation of ceftiofur sodium-liposome.1 In order to control the quality of ceftiofur sodium, the methods for determination of ceftiofur sodium quantity by ceftiofur sodium and for determination of entrapment efficiency of ceftiofur sodium liposome by sephadex gel filtration chromatography-ceftiofur sodium were established, which could determine ceftiofur sodium quantity and entrapment efficiency accurately.2 Select the method, the technology and prescription. We use the stability, recovery ratio entrapment efficiency as the estimating indexes evaluating reversed-phased method, ether injection method, membrane method. The result showed that reversed-phase method poses the best entrapment efficiency and better stability in all of the methods. We evaluate the technology of preparation .The result showed that when the temperature of water is 25℃, we can gain optimum preparation. We estimate the factor of prescription. The result showed that when the Ch:PC is 3:1, ceftiofur sodium to(Ch+ PC) is 1:20, ceftiofur sodium solution to chloroform is l:3, pH is 6.5.We can gain better encapsulation and better stability ceftiofur sodium-liposome. Its entrapment efficiency is 62.76±1.09%.3 Quality evaluation of ceftiofur sodium-liposome. We estimate quality evaluation according form, practical size, stability, release property in vitro and leakage. The liposome is milk and not impurity in our eyes. The structure of ceftiofur sodium-liposome was observed with transmission electron microscope is like a ball or like a vesicle. The result shows a better normal distribution, liposome can storage 60 days in 4℃. Absorptionrate constant of ceftiofur sodium-liposome in vivo is 17.43% in 24 hour, the absorption rate constant of ceftiofur is 100% in 5 hour.4 Ceftiofur sodium liposome, was compared its MIC against E.coil, streptococcus, pasteurella multocid and staphylococcus aureus with free ceftiofur sodium in order to study its antibacterial effect in vitro. The MIC of ceftiofur sodium liposome against the bacterias mentioned above was 1/4,1/4,1/2,1/4 of that of free ceftiofur sodium. The results showed the antibacterial effect of ceftiofur sodium liposome was superior to free ceftiofur sodium.