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Study On Two Kind Of Bacterial Diseases Of Cultured Turbot

Posted on:2008-07-14Degree:MasterType:Thesis
Country:ChinaCandidate:S F WanFull Text:PDF
GTID:2143360218458423Subject:Animal breeding and genetics and breeding
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Turbot (Scophthalmus maximus) was firstly introduced into China by Yellow Sea FisheryResesrch Institute in 1992. After achieving technological breakthrough on the fry domestication,broodstock rearing, artificial seed breeding and compond feeds, the fish was extensively culturedalong the coast in north China. However, due to the high stocking density, poor management andthe susceptivity to disease during larval stages, diseases occurred frequently. They caused highmortalities and reduced the seed outputs of hatcheries.pronounced unilateral exophthalmia investigation from 2005 to 2007showed that Floccosoidsfrom Bristles and exophthalmia diseases commonly occurred,. they were caused by bacterialagents resulting in death.The source thorn floccosoids gets sick the main symptom is the bristles changes pale white,The organization around the bristles also starts to infect, The floccosoids taking form, somefloccosoids connect into a slice .Lower jaw of disease fish soft tissue hyperaemia red and swollen,also may fester to be changed into white. Infect big weight water chestnut left-eyed floundersabove 0.25 kilogram mainly, 4 months or so reaches infection later stage, the number day innerillness spreads comparatively quickly, the death rate reaches 100% now, by now, not find in litter,this disease discovers first, does not have relevance report in home and abroad category.Bacterium separation gets advantage bacterial strain WF-1 , WF-2, they are germ from dieasebody by taking over infection test. That organization pathological mechanism analysis indicatesthe infection that pathogenic bacteria leads to is the whole body's, Liver of disease fish, locationsuch as spleen, kidney, the brain and the heart all have lesion to varying degrees. By the fact thatdyeing, light microscope, electron microscope, physiology biochemistry testing, 16 S rRNAgenes arrays analysis testifying WF-1 is the staphylococcus horninis, WF-2 is the PseudoalteromonasThe disease fish simple eye double suffering from the protruding eye ailment protrudes,whose eye socket vicinity organizes hyperaemia, dropsy displays Dan red, crack, bleed with thefact that the state of illness going a step further developing, may violence happened in the fish eye;Disease fish maxilla part occurrence swelling, hyperaemia rubedo; Area accompanies gill coverto have hyperaemia to rubedo also sometimes phenomenon, grave person many place bleedingspeckles appears on meeting; Disease fish has the ascites phenomenon. Fish shows a disease forchronic death. Bacterium separation gets advantage bacterial strain W-3, infecting demonstrationafter return is protruding eye ailment pathogenic bacteria, W-3is Enterococcuscasseliflavus leadintestines coccus by the fact that dyeing, light microscope, electron microscope, physiologybiochemistry testing, 16 S rRNA genes arrays analysisThe present study is domestic staphylococcus homnise early Scophthalmus maximus diseasefirst time report. Has carried on 20 kind of common medicines antibiotic sensitive experiments to the above main pathogenesis bacterium. The medicine develops which in this foundation hascarried on the experimental clinical test, proved gets sick to the source thorn floccosoids with theraised exophthalmia diseases good preventing and controlling effect.
Keywords/Search Tags:Turbot(Scophthalmus maximus), Bacteria identification, Floccosoids on Bristles, exophthalmia
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