Test Of Allergy Induced By Classical Swine Fever Vaccine On Guinea Pig

Allergy, as well as named anaphylaxis or hypersensitivity, is abnormal or pathological immunization reaction when organism is stimulated by some antibody. It often displays violent responses of some tissues or organs, even system, and revoke various functional disturbance. Typeâ… allergy, as well as called immediate hypersensitivity, is often encountered in clinic. In practice, there were some unexpected deaths when animal were injected vaccine, especially injected CSF vaccine. Some reports inferred this phenomenon was typeâ… allergy which was caused by CSF vaccine. This research introduced guinea-pigs which were easily to stimulate as experiment animal models. Through clinical simulation of allergy after injecting CSF bovine testicle cell vaccine, we certified this idea exactly. Bovine testicle cell, CSFV and calf serum can arouse allergy in CSF vaccine, and this kind of substance must be antigen or haptene. To look for the real allergen in CSF vaccine, the allergy tests aimed to main ingredients of CSF vaccine were conducted. Through clinical observation whether or not to revoke allergy or degree of allergy, allergen was certificated. Allergy is affected by a great of factors. In addition to allergen and body constitution, provocation ways are also important factor. In order to find the main way which can cause systemic allergy, comparison tests of four injection ways of sensitization and stimulation were carried out in our test. At the same time the levels of serum IgE were measured by ELISA after sensitization and stimulation. These can be used as foundation for study allergy further.The tests results as follows: (1)This research introduced guinea-pigs as experiment animal models successfully. It proved that CSF vaccine may cause allergy. Group experiments revealed that calf serum was the most important allergen, classical swine fever virus was the second and the bovine testicle cell was the last. (2)Through comparison of different injection ways of sensitization and stimulation, it showed that all injection ways could cause sensitization, but only the intravenous injection induced serious allergy when stimulated. (3)IgE were measured by ELISA after sensitization and stimulation. Compared with the control group, there was no significant difference on the levels of serum IgE.