Effect Of Main Components From Semen Vaccariae On The Function Of Mouse Mammary Epithelial Cell In Vitro

Disadvantages such as low lactating, high incidence of diseases are existing in milk cowbreeding of our country. For a long time, people use antibiotic and some chemic compound as feedadditive to prevent and cure diseases of milk cows, so as to improve lactating of milk cows.Nevertheless, these additives have great side-effect, a lot of remainder. It is easy to bring drug-fastand cause abnormality, cancer and mutation, which jeopardize body health. Chinese traditionalmedicine is derived from nature. It has a lot of advantages such as low side-effect, little remainder,and hardly bring drug-fast, tt has applied abroad in milk cow breeding of our country. ManyChinese traditional medicine ant its compound which have the function of unblocking blood vessel,recruiting vigor and accommodating spleen and stomach can improve lactating remarkably. SuchChinese traditional medicine as liquorice, Semen Vaccariae, and etc contain a lot of nutritioussubstance and biologically active substance. They can supply organism with nutrition, improvecreatural appetite, digesting and assimilating, metabolism, blood circulating of organism. They alsocan kill microorganism, improve immunity of organism, prevent and cure diseases, accordinglyimprove lactating capability of milk cows.The Chinese traditional medicine Semen Vaccariae applied widely in milk cows breeding ofour country as additive. It has the function of unblocking blood vessel, inducing lactating,detumescence an dwindling scar and so on. Its main components are saponin, flavonoid glycosideand coumarin.This test studied both the extracting methods of three main components from SemenVaccariae—saponin, flavonoid glycoside and coumarin and the identification using chemicmethods and chromatogram methods. Add these components to cell cultural medium with severalconcentration. Then detect their effects on multiplication of mouse mammary epithelial cells usingthe mothod MTT and the expression of beta-casein of mouse mammary epithelial cells usingReal-time PCR. Furthermore, this test put up pilot study of their mechanism. We detected theexpression of cyclin D1 and phosphatic stat5 using laser scanning confocal microscope. Finally, the function of the effective component was validated through animal experiment.The result indicated that the component of saponin could restrain the multiplication of mousemammary epithelial cells (P＜0.05), and its function was stronger as its concentration(50～200mg/L) increasing. The component of flavonoid glycoside could promote themultiplication of mouse mammary epithelial cells (P＜0.05), and its function was stronger as itsconcentration (100～200mg/L) increasing. Whereas the component of coumarin had no influenceon the multiplication of mouse mammary epithelial cells (P＞0.05).The component of flavonoid glycoside was able to promote the expression of beta-casein (P＜0.05). The component of saponin and coumarin had no influence on the expression ofbeta-casein (P＞0.05). The component of flavonoid glycoside could promote the expression ofcyclin D1 and phosphatic stat5 (P＜0.05). This indicated that the component of flavonid glycosidecould promote the multiplication of mouse mammary epithelial cells by activating the expressionof cyclin D1 and regulate expression of beta-casein by activating Jak-stat5. Animal experimentindicated that the component of flavonoid glycoside could increase lactating of mouse (P＜0.05).