Effect Of Different Matrix Factors On Rhizobium-alfalfa Symbiotic Match

The alfalfa is one perennial leguminosae herb, the economic value and the nutritional valueare high, has "king of the forage grass" the laudatory name; The alfalfa vaccination with it matchhighly effective root nodule bacteria, can enhance the nitrogen fixation efficiency and the adultplant quality, the festival fat production increase, has the extremely important ecology significance.This research has carried on some discussions on the alfalfa root nodule bacteria in the differentmatrix with the alfalfa variety paragenesis match, selected 3 alfalfa variety to vaccinate separately11 different root nodule bacterias, under greenhouse condition, through decency coal, vermiculiteand pearlite double-decked earthen bowl cultivation experiment and soil pot experiment, By theaerial parts dry weight, the total nodule number, the aerial parts adult plant highly, targets and so onchlorophyll content as well as entire nitrogen studies 3 alfalfa variety to vaccinate separately thedifferent root nodule bacteria growth effect, screens has the best paragenesis effect root nodulebacteria strain to each alfalfa variety, then will screen after the highly effective strain to carry on thefield examination.The experimental result is as follows:1. Makes the decency coal, the pearlite and the vermiculite carries on between the root nodulebacteria and the alfalfa variety match as the matrix, alfalfa's nitrogen fixation effect and thegrowing trend is higher than other two kind of matrix, the decency coal may first take the highlyeffective strain screens matrix. Its paragenesis effect is in turn makes the decency coal＞thevermiculite＞the pearlite.2. In the decency coal cultivation experiment to the entire nitrogen, average high, the totalnodule number and the chlorophyll and the aerial parts dry weight carries on the relevant analysis,obtains the entire nitrogen and the adult plant aerial parts dry weight assumes the correlation to beextremely remarkable relates; The chlorophyll and the dry weight assume the correlationremarkable relations, but average Gao Hezong the nodule number and the aerial parts dry weightassumes the correlation not obviously to relate. Therefore, conducts in the paragenesis matchresearch in the decency coal matrix to be possible to use the dry weight, the entire nitrogen and thechlorophyll content evaluates the paragenesis effect, but the total nodule number and average plantheight is a norm for reference.3. Uses the soil bonsai to carry on the root nodule bacteria and the alfalfa highly effectiveparagenesis match experiment, in the simulation production practice the big field soil environment,conducts the research to the root nodule bacteria in soil compatibility. Screen the result through thesoil screening result and the decency coal the relevant analysis, the correlation coefficient is the 0.29426, pH value is more than 0.05, therefore, screens with decency coal screening and the soilresult existence difference.4. Screen with the decency coal and the soil bonsai match the best four root nodule bacteriaswith the alfalfa variety giant 201 paragenesis to carry on the field plot vaccination experiment,finally indicated, both result is consistent. Although in the soil includes the indigenous root nodulebacteria, but all can enable vaccination processing to achieve an obvious production increase andthe improvement quality effect. With the giant 201 alfalfa varieties match highly effective nodulestrain is SM29, SM32, SM33 and SM35, after vaccinates above four kind of strains, entire nitrogencontentequally grew compared to the comparison 27.38%; A high comparison equally grew60.27%; The aerial parts dry weight grew 2～3 to double, separately grew 327.00%, 300.65%,280.56% and 279.70%; After the vaccination fresh weight and the comparison compares alsohas theremarkable enhancement, separately grew 148.12%, 132.64%,121.34% and 121.76%. After thevaccination obviously enhanced the nitrogen fixation effect and the biomass, may further investthese highly effective strains into the actual production in.5. For tries the root nodule bacteria in four the physiological characteristic aspect, they havecertain resistance to the chloromycetin and the ammonia animal pen penicillin.In the acid-proofalkalinity aspect, strain SM29 and SM35 bear the acid and alkali the scope between 4.0～10.0,Explained two fungi when field application, can adapt the strong soil pH change, but when the pHvalue is lower than 4, two kind of fungi cannot the very good survival. In anti-salinity aspect, theSM29 anti-salt may reach 5%, SM35 may reach about 4%,Can adapt to the high salty soilcircumstances.