| Rapeseed(Brassica napus L)is one of the most import.ant industrial crops in theworld. However, this crop has been suffering from the destructive disease of Sclerotiniasclerotiorum for a long time. A fter long time practice, no completely resistant cultivarshave been identified in rapeseed, as well as in the closely related species. Resistance toS.sclerotiorum in rapeseed is a quantitative trait and easily affected by environmentcondition. Till now, there is no widely accepted identification method for the resistanceto S. sclerotiorum. These questions make breeders difficult to select really resistant plants.Fortunately the success on plant genetic engineering has brought light to the rapeseedbreeder. Oxalate oxidase (OXO), a class of detoxification enzyme, catabolize oxalic acid(OA) and O2 into CO2 and H2O. Based on evidence that oxalic acid is involved in thepathogenicity of many Sclerotinia species, our objectives were to recover transgenicrapeseed expressing an oxalic acid-degrading oxalate oxidase, and we hope oxalateoxidase can confer enhanced resistance to S. sclerotiorum in rapeseed.The plant binary expression vector GBBS was constructed harboring the germingene arid plasmid B7, driven by CaMV 35S promoter. GBBS plant expression vectorwere used for Agrobacterium-mediated transformation of rapeseed 98C40 and Westar. Atotal of 22 independent transgenic plants have been obtained, including 7 from 98C40and 15 from Westar.
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