Study In Inhibitory Metabolic Products Produced By Bacillius Firmus

the research was in order to deeply explore the producing condition, the inhibitory mechanism.the inhibitory component of metabolic products produced by Bacillius firmus.1. The research is about the inhibitory culture medium and growth condition. From thebiology determine, the NB culture medium is confirmed as the best culture medium. Then thebest culture condition was determined by orthogonality test. It contains different carbon,nitrogen, temperature, pH, and oxygen, which is 8 g of beef extract, 10 g of peptone, 14 g ofglucose, pH7.5, 150 ml culture liquid shaky cultivate in 300 ml triangle bottle, and the fittesttemperature is 35℃. It is showed that the inhibitory rate is above 79% by metabolic productsof Bacillius firmus growed on the best culture condition, and no conidiophore had beenproduced. Otherwise, the upgrowth curve of Bacillius firmus and the inhibitory activity curveof metabolic products has been mensurated. It was infested that the highest quantity ofBacillius firmus and the best inhibitory activity was appeared at 60 hours cultivate. Generally,the cnltivate time conldn't over 72 hours, or else, both the quantity of Bacillius firmus and theinhibitory activity were turn into wane. It would make against on distilling and utilizing.2. The researches in this part were about the inhibitory mechanism and extension of metabolicproducts. The inhibitory mechanism is dissolution and counteraction. Which were confirmedby the effect of metabolic products to the mycelium and conidiophore of Pestalotiopsisfunerea. The color of mycelium turn dark and the wall was turn thick. The septum ofmycelium increased, and each section turn to ellipse. The hardest one was divarication, themost of which was conglobate, contains roundness grain in it, indeed broken, and thebioplasm spill over. Some mycelium has been ruptured, cracked, and turned abnormality, thebioplasm spilled empty. The mycelium of Pestalotiopsis funerea couldn't born fromconidiophore. The cell wall of conidiophore has been turned thin in evidence, especially forthe first cell, which has been turn big, the wall was thiner than the other two cell's, indeedturn like a purse. The third cell of conidiophore has been turned like a purse at the same timepartly. After 48 hours, the third cell or the first cell of conidiophore have been broken, the bioplasm was spilled over. The study on the inhibitory range was indicated that, the range ofthe inhibitory effect by metabolic products, which produced by Bacillius firmus. It manifestedthat the metabolic products has obvious inhibitory effect to kinds of pathogeny, except Mucorracemonus. The best effect was to Sclerotinia sclerotiorum, it makes fungus couldn't grow.The wrost was to sweet-scented osmanthus husk festered fungus, but the inhibitory rate wasalso above 40%.3. The researches was progressed on depositing gradely by vitriol ammonium, dialysis,Anion-exchange Chromatography, SDS-PAGE. The aim is to make sure the character andmolecular weight of its metabolic products. It was obvious that, the metabolic products woulddeposit in the different saturation of vitriol ammonium, and the more vitriol ammonium, themore deposit. The brown deposit float on the water, when the saturation of vitriol ammoniumarrived at 70%, both the inhibitory activity and the quantity of protein reach the crest. Thedialyzed deposit also have inhibitory activity, and it was steady in ultraviolet radiation,chloroform and trypsin, but not totally steady in high temperature, acid, and proteinase K.After Anion-exchange Chromatography, three kinds of sample has been collected, but only asample has inhibitory activity. Both of them were made use of SDS-PAGE. It was ascertainedthat the sample which has inhibitory activity bring a clear cincture, although the cincturewasn't clear, the molecular weight could be confirmed to 29KD more or less contrastd withprotein marker.