The Polymorphism Of Interferon-tau Gene And Their Relationship With Reproductive Hormone And Conception Rate In Chinese Holstein

This experiment did research on the sequence of Chinese Hostein interferon- taugene CDS firstly by using the PCR production Direct sequencing technology. Basedon the detection of registered data, the effects of different genotypes with conceptionrate,the different deuto foetus with progestone level and estradiol level, pregnancycondition with progestone level and estradiol level were analyzed.1.There are two mutation locuses in interferon- tau gene CDS of the Chinese Hostein,they are T→C mutation and C→T mutation. besides that zygotic genotypes aresuperiority genotypes in different mutation.2.Heterozygosis degree of the different mutation locuses is 0.7430 and 0.8350; Thepolymorphisms information content is 0.3700 and 0.3680, so mutation locus one andmutation locus two are midrange polymorphisms. The results of x~2 fitness test of locusone and locus two indicated that the Chinese Hostein were high significantly biasedfrom Hardy-Weinberg equilibrium (P＜0.01).3. least squares analysis showed that the different locus genotype is quiet with theChinese Hostein conception rate (P＞0.05).4. Least squares analysis showed that level progesterone of the Chinese Hostein issignificant devitation (P＜0.05) in 0 foetus and 4 foetus, 0 foetus and 5 foetus, 1foetus and 5 foetus and 2 foetus and 5 foetus.Least squares analysis showed that levelprogesterone of the Chinese Hostein is extreme significant devitation (P＜0.01) in1 foetus and 4 foetus, 2 foetus and 4 foetus, 3 foetus and 4 foetus, 4 foetus and 6foetus, 5 foetus and 6 foetus and 6 foetus and 7 foetus. Least squares analysis showedthat level estradiole of the Chinese Hostein is significant devitation (P＜0.05) in 0foetus and 2 foetusang and 1 foetus and 2 foetus. Least squares analysis showed thatthe different pregnancy condition is quiet with level progesterone and estradiole in theChinese Hostein (P＞0.05).