The Toxicities And Pharmacokinetics Residues Of Praziquantel On Goldfish(Catassiusauratus) By Oral And Bath Treatment

A series of exiperiments were carried out for studies on the toxicities and Pharmacokinetics Residues of Praziquantel on goldfish(Catassiusauratus) by oral and bath treatment. At first, the acute and subacute toxicities were investigated in goldfish(Carassiusauratus) by oral and bath treatment. The results showed that the 24,48,96h LC₅₀ for goldfish were 54.80,51.07,50.78mg/L espectively, the safey concentiation was 14.29mg/L by bath treatment, while Praziquantel had no obvious lethal effect on goldfish with LC50＞10g/kg by oral treatment. With regard to the subacute toxicity test, The activities of ALP in the serum had no obvious change by oral and bath treatment, when the Praziquantel concentration were 100mg/kg,2.54mg/L the activities of SOD and ALT had no obvious variances. The activities of SOD were induced remarkably and then restored dradually when praziquantel level was at 400mg/kg while there was a adverse when Praziquantel level was at 10.15mg/L. The ALT activities were significantly increased continunously when the praziziquantel concentration were 400mg/kg and 10.15mg/L.Secondly, A high performance liquid chromatographic method with fluorescence detection for the determination of Praziquantel residues in plasma and tissues were developed. Analysis was performed on a WatersC18(5μm,250mm×4.6mm)with acetonitrile:water(1:1 v/v) as the mobile phase. The column was kept at room temperature, and the flow rate was kept constant at 0.9ml/min.The fluorometric excitation and emission wavelengths were set at 265 nm and 280nm, espectively. The correlations of calibration curve were all good, which correlation coefficient were more than 0.9990. The limit of detection (LOD) was 0.01μg/mL,0.01,0.02,0.01,0.01,0.02mg/kg in plasma,muscle,skin,liver,kidney,gill espectively. The average extraction recovery was more than70% from skin and more than 80% from other tissues and plasma. The intra-day coefficient of variations and inter-day coefficient of variations were less than 10%.Trirdly, pharmacokinetics and residues of Praziquantel on Goldfish by oral and bath treatment., under 22±1℃, Plasma and tissues samples were collected at different intervals. The samples were determined by using reversed-phase high performance liquid chromatography. The concentration-time data of praziquantel in plasma were analyzed with 3P97 computer program. Following single oral administration, the plama concentration-time data of praziquantel were best described by a two-compartmental open model with absorption, C=4.47614.4761e^-0.6477t+2.7801e^0.7121t-7.2562e^-1.96645t Distribution and elimination half-lives of 0.3525,1.0701,9.7335h, respectively. The time to peak plasma concentration, T_p was 1.1277h and peak concentration (C_max) was 3.94ug/mL. The procession of praziquantel after bath treatment was fit the one-compartment open model, pharmacokinetic equation: C=0.4094(e^-0.1502t-e^-6.9977t). The main pharmacokinetic parameters of cyadox in carp were as follows: T_1/2Ka0.0991h,Tp0.5610h,C_max0.3683mg/L,Vd/F2.6673mg/mg,T_1/2ke 4.6149h. Pharmacokinetic analysis of the plasma concentrations showed that praziquantel exhibited a rapid absorption and distribution, but slow elimination. The concentrations of p raziquantel were highest in liver and kidney by oral treatment while in gill by bath treatment. Following oral and treatment, praziquantel was detected in skin until 96h and 24h after treatment.