Study On Genetic Relationship And Molecular Identification Of 38 Tea (Camellia Sinensis (L.) O. Kuntze) Cultivars By SSR Markers

Tea plant (Camellia sinensis (L.) O. Kuntze) is one of the important economic plants in China, which had long planting history and distributes widely. It has been proved that Southwest of China is the origin of tea plant, having rich of tea resources.Currently, two germplasm resources of tea plant had been set up in China where 3300 individuals were collected. The abundance of tea plant provided rich resources for the breeding new cultivars. Microsatellites or single sequence repeats (SSR), a newly developed molecular marker techniques, had been recommended as standard markers because they were highly polymorphic, co-dominant and reliable.In the study, total 38 tea cultivars samples, which origin in Sichuan,Fujian and so on were used to get genomic DNA to analysis the genetic relationship of the different gene pool by SSR markers. The main results were briefly summarized as follows:1 Six pairs of SSR primers out of sixteen were identified having polymorphism bands. Total of 61 DNA bands had been amplified using these polymorphic markers to detect 38 tea cultivars. Among which 61(100%) were polymorphic,the average number of DNA bands amplified by each primer was 10.1, the most was 13 and the least was 8. The simple matching coefficient of similarity was between 0.557 to 0.902, on an average 0.710.2 Based on genetic similarity coefficient, the UPGMA dendrogram of 38 tea cultivars was made by NTSYS cluster analysis. At similarity coefficient of 0.693, all the accessions could be clustered into four groups. Longjing43,Mengshan9 and Maolv were classified into groupâ… ; Longjingchangye,Qingfeng,Yingshuang,Anjibaicha,Biyun,Pingyangtezao,Wuniuzao,Zhe'nong 117,Fuding,Anxishuixian,Yuanxiaocha,Zhenghedabaicha,Fudingdahao,Meizhan,Fuxuang,Xicha5,Xicha11,Fuzao2,Donghuzao,Zhuyeqi,Mingshan213,Mingshanbahao,Nanjiang4,Qianmei303,Qianmei502 and Taiwandaye were classified into groupâ…¡; Shuyong307,Mingshanzao,Mengshan23,Mengshan11,Qianmei419,Yinghong2,Hainandaye and Huangyeshuixian were classified into groupâ…¢; Fujianshuixian was classified into groupâ…£alnonely.3 By the amplication products from genetic DNA obtained in the study ,34 tea accessions could be identified. The results indicated that SSR was a useful tool for molecular identification of tea accessions.