Asses Function Of Hydrolysate From House-flies Larva Protein And Study On Solid Beverage Formula

House-flies, belonging to the family Muscidae of Diptera. House-flies larva is a kind of highly nutritious protein resourse and the essential amino acids are complete. The nutritional evaluation of the amino in House-flies larva was testificd. Having the easily absorption characteristic, these low peptide still have many good functions characteristic and the physiological activity. They can be used widely in the food field. The paper was aimed at studying the techniques of enzymatic hydrolysate of House-flies larva, and asses the hydrolysate's function, according that studying the formula of peptides beverage in order to develop the new protein resourse.1. The technics of hydrolyzationFirst, the performance of enzymes was compared. With the index of DH and the sensory assessment, Alkaline proteinase was selected as the suitable protease to prepare hydrolysate. Simple factor analysis and orthogonal design L₉(3⁴)was adopted to optimize the conditions of hydrolysis. The result prefered under the condition of temperature 55℃, pH 7.6, ratio of enzyme to substrate (E/S) 3.0%, ratio of water to substrate(w/v)1:5 and optimal time of hydrolysis was 5 hours. The L₉ (3⁴) Experiment indicate that the order of impantance is pH＞E/S＞w/v＞temperature.2.The assessment of nutrition and active functionAfter the acute toxicological experiment, the LD₅₀ of hydrolysis of House-flies larva was＞30g/kg·bw, and it's innoxious.The intake and avoirdupois after thrity days'feed increased batter than the blank by feed low dosage. All experiment groups had the uptrend about apparent protein digestant rate, the difference between experiment group and black was not significant. After feed the low dosage and medium dosage, the total counts of coliform bacteria of feces could decreased and the counts of·Lactobacterium increased significant. And the low dosage had prominent stimulative effect on bowels' growth.In the exprimental research on hydrolysate's active effect, the low dosage and medium dosage could prominently enhance the exhausive sports time, and had prominent stimulative effect on SOD. The low dosage group also had remarkable advanced CKactive and LDH active. The LD content in blood had parallel level.3. Methods of debitter and mensuration of characters Activated carbon and different sorbents were chosen to debitter hydrolysate. Experimental design included single factor, orthogonal design, combination analysis. The result showed that the optimum conditions and optimum method of debitteeing peptide as follows:β-CD3.0%, temperature 65℃, time:40min. Ater disposal, the water-solubility of hydrolysate is more better than the larva protein.4. The studies of peptide beverageThe studies of peptide beverage was based on hydrolysate of larva protein,and the conditions of homogenization is 65℃, 45 MPa. The formula of nutritive beverage was composed of xanthic acid 0.1%, alginate 0.05%, sugar 17.65%, citric acid 0.29% and pineapple essence 0.1%, under these congditions the sensory quality and stability of products was optimum.