The Study Of E.suis On Physiological And Biochemical Variables In Pigs And Culture Experimen Of E.suis In Vitro

There are two experiments in this study: one is for the changes of some biological andhematological variables in blood of pigs infected with E.suis. The other is for the cultureexperimen of E.suis in vitro on the normal red cells of eight kinds of animals and person.The first research explored the changes of some biological and hematologicalvariables in slight or grave Eperyhrozoonosis by observation on Eperythrozoon withdirect microscopy, clinical symptom, hematological change and the physiological andbiochemical indexes. The results showed as fellows:Compared with those of control, the content of MCHC and WBC in blood in group ofslight infection were significantly higher (P＜0.05), the RBC, HGB and MCH contentdidn't changed obviously (P＞0.05), the activity of ALT and CK increased significantly(P＜0.05 or P＜0.01), the activity of Glu decreased significantly (P＜0.05), the activity ofAST and LDH didn't changed obviously (P＞0.05); the content of MCH MCHC and WBCin blood in group of grave infection were significantly higher (P＜0.05or P＜0.05), thecontent of RBC and HGB decreased significantly (P＜0.01), the activity of ALl, AST, CKand LDH increased significantly (P＜0.05 or P＜0.01), the activity of Glu decreasedsignificantly (P＜0.01 ).The results demonstrated that E.suis could result in the obvious changes of thebiological and hematological variables in blood of pigs.The second research study to cultivate Eperythrozoon suis in vitro on the normal redblood cell of eight kinds of animal and person for checking the infection of the diffent redcells on E.suis.The results indicated that culture with different red cells in vitro, E.suiscould grow and regenerate in the complete medium. E.suis was infectious to these ninedifferent red cells. Among these, the cells of pigs had the most susceptibility and thehighest infection rate which was up to 99%. The red cells of pigs could generate 36generation in the successive culturing. For others, the red cells of bovine and goat couldbe cultured 24 generations, the five red cells (sheep, dog, mice and human) could becultured 18 generations, and rabbit can be cultured 9 generations, human and dog can becultured 7 generations, sheep and fowl red cells be cultured generations.The purpose ofthis study is to determine the kinds of animals which are infective to E.suis, by which wecan take measures with drug for the infective animals to be prevented from disease.