| The staphylococcus is a kind of main contagion cause germ which causes milk cow mastitis. Experiment to shout with specially Huhhot area, through CMT and body cell examination assurance of 223 samples from 77 mastitis cow .Carried out the authenticate of separation staphylococcus ,cure evaluate and the medicine sensitivity experiment. Also studied the disease staphylococcu to carry on to RAPD random to 63 stubs separated.The conteracting toxic substances experiment shows 63 kind stubs is pathogenicity staphylococcus.The experiment use 9 major type 21 kinds of medicine which is clinical treatment on pathogenic staphylococcus. penicillin, the sulfasulfonamide medicine and cephazoline. the medicine all have already produced different degree drug resistance ,but aminoglycosides and Du-6859a have obviously bacteriostasis effect.After RAPD found that 63 strain staphylococcus has muti-band froms, expanding between 2~13, molecular weight is between the 50~2200 bps.Use SPSS11.5 analyze the staphylococcus band , according to genetic distance cluster analysis the result show 12 mainly gather types ,and 73% genotypic hasgenetic relationship.Therefore, this experiment passes drug sensitivity experiment, and RAPD technique, studied huhhot area staphylococcus drug resistance and different genetic gene ,and comparison with domestic and international the genotype.Foundation for research of drug resistance gene and better DNA vaccine. |
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