Cloning And Expression Igλ Of Bcell Of Chicken

Infectious bursal disease virus (IBDV), a member of the Birnaviridae family, is a pathogen of infectious bursal disease and causes a highly contagious and severe immunosuppression by destroying B-lymphoid cells presented in the bursa of Fabricius, leading to an increased susceptibility to other pathogens and considerable economic losses in poultry industries worldwide. The genome of IBDV consists of two segments of double-stranded RNA (A and B). Segment B encodes the VP1 protein, the putative viral RNA-dependent RNA polymerase and segment A contains two partially overlapping open reading frames (ORF). The smaller ORF encodes VP5, a nonstructural protein of 17 kDa. The larger ORF encodes a polyprotein precursor with a molecular weight of 110kDa, which is proteolytically cleaved to produce the structural virus protein of VP2, VP3 and VP4. These proteins form the viral capsid and represent the B cell epitopes inducing the neutralization antibodies.Receptor is a giant molecule substance. It lies in adipose membrane or cell and it can bind signal substance out of cell. And at the same time provoke biological effect. Receptor likes a door when cell receive stimulus. Receptor is the first link when educe accommodation function. Receptor can be divided four kinds basic type, enzymatic activity receptor, nuclear receptor, ion passage receptor, G proteinum receptor. B cell receptor first is binded when infectious bursal disease virus to make incursions into chickens body.This research amplification B cell Igλ. gene by gene engineering principle. I construction procaryon express vector pET43aSl of B cell Igλgene, we induce express proteinum of this gene. In order to carry out correlation experiment, we make B cell monoclonal antibody, meantime ,we construction pcDNA3 eucaryon express vector. We gain proteinum in cos7 cell.This proteinum to establish a foundation for other experiment.In this study, procaryon express proteinum can response with His-Taq monoclonal antibody,This indicate Igλgene encoding proteinum is expression succeedly. The consequence of eucaryon express proteinum binding with IBDV,then by fluorescence staining, detection on flow cytometer(FCM), we can gain a conspicuous discrimination result. This result manifest IBDV and eucaryon express proteinumpossess affinity. Thereby, mechanism of IBDV infection B cell is certificated initial.Invasion mechanism is to explain in gene level, This study settles fundament for IBDVpreservation and Pharmaprojects.