Identification Of Resistant Resources, Inheritance Of Resistance And Microsatellite Tagging Of Resistant Gene To Soybean Mosaic Virus In Soybean

Soybean mosaic disease, caused by soybean mosaic virus (SMV), is one of diseases in soybean production worldwide. It causes yield loss and seed quality deficiency seriously. breeding resistant varieties is the most efficient method to control SMV. The success and failture of breeding resistant varieties depend on resistant resources and the realization of inheritance mechanisms of resistance to SMV strains. In order to provide resistant sources for breeding resistant varieties and lay a foundation for molecular marker-assisted selection and fine mapping and map-based cloning of resistant gene.The major objectives of present study were to screen out resistant sources, study the inheritance of resistance to SMV and identify the microsatellite markers linking to the resistant gene.The research showed that after 161 soybean varieties were inoculated with SC-3, SC-8, SC-11, SC-12, SC-13 and SC-17, eight cultivars (Ji B04-3, Weidou 6, BN101, Hengsheng 1, Bindou95-20, Zhongpin02-046, Dongda 2 and Dongda 4) were resistance in infection to six strains, which represented 4.94% of the total varieties. Seven cultivars (Youchun 01-32, Gongdou 114-1, Zhongdou 32, Ji G04-85, Zhongzuo 00-683, Ke 02-17 and Zhongpin 661) were resistance in infection to five strains, which represented 4.32% of the total varieties. In addition, six and eight cultivars were resistance in infection to four and three strains respectively. Six cultivars (Zhongzuo 01-03, Meng 89-52, Shanning 11, Jinghuang 03-5, Chengdou 7 and Nandou 99) were resistance in development to six strains. These cultivars can not only be used directly in soybean production, but also be used as resistant resources in breeding programs. The study also showed that cultivars from Huang-Huai-Hai valleys were more resistant to SMV than those from the other regions.SC-8 and SC-11 are all prevalent strains in the middle and northern Huang-Huai region and northern China spring planting soybean region. The study of inheritance of resistance to SMV showed that a dominant gene controlled the resistance to SC-8 in Qihuang NO.1, Qihuang22 and PI96983. The resistant genes in Qihuang NO.1 and Qihuang22 were alleles, but the resistant genes in Qihuang NO.1 and Kengfeng NO.1 maybe not at same locus. The result also showed that a dominant gene controlled the resistance to SC-11 in Qihuang NO.1, The resistant genes in Qihuang NO.1, Qihuang22, Kwanggyo and Zaoshul8 were alleles. The reactions of F_2:3 of Qihuang NO.1×Nannongl138-2 were identified contemporaneously when inoculated with SC-11 and SC-14. The result indicated that R_SC-11 and R_SC14Q maybe at same linkage grop.Four SSR markers Satt114, Satt334, Sat₂34 and Sct₀33 were found to be closely linked with R_SC-11 by the bulked segregant analysis. R_SC-11 were located in F linkage group according to the joint map by Song(2004). Then 18 primers located in F linkage group of the joint map and expressed polymorphism between two parents were used to constructed the F linkage group. The length of F linkage group constructed in present study is 245.8cM and average distance between two markers is 13.41cM.