Research On Producing Sugar By Fermenting Maize Stalk In Simulated Internal Environment Of Rumen

The rumen of a ruminant animal is quite unique peptic in the structure of nature; its main characteristic is that it can digest the massive crude fiber materials that other animals are not able to digest. As a result of this characteristic, the rumen structure digestive mechanism has always drawn a lots researcher's attention. The present research has discovered that it has massive microorganism, including bacterium, fungus and protozoan, which can secrete the cellulase, just because the rumen of ruminant animals can digest the textile fiber materials. It is mainly the cellulose function that caused the rumen of ruminate animals to utilize the crude fiber. If we can apply the rumen microorganism fermentation crude fiber material mechanism in vitro fermentation as well as the industrial production, which could be brings a greater income not only for the agricultural production but for the increase of the farmer income, even the resolution of the crops straw stalk processing problem. In view of the utilization of the rumen function superiority; this article conducts the research on the rumen microorganism as well as the simulation of fermentation on corn straw stalk production sugar to the rumen microorganism under the laboratory condition.There are a lot of animalcule inside rumen, including bacteria, epiphyte and protozoan. After a sufficient study on relevant research conducted in China and abroad, this paper work carried on experimental research in the biological characteristics of cellulolytic fungus in ox rumen, gastric juice of which was provided by Jilin Haoyue Group. Also, the parameters which affect the extracorporeal fermenting have been analyzed in this work.To begin with, the cultured rumen animalcules have been confirmed to be ruminococcus albus after macroscopic identify, and their growing time was 96 hours. By calculating the coccus on the culture medium surface, we knew there were 1.27×105 white ruminococcus albus per milliliter. Upon this work, the growing area has been calculated, so that the growing speed could be calculated. Subsequently after comparing the calculated results with each other, 10 times diluent rumen gastric juice has been confirmed suitable to afterward fermenting experiment, with 1010 quantitatively coccus in it; 1000 times diluent rumen gastric juice has been confirmed suitable to experimental rumen animalcule expanding culturing. This research was the key part in this dissertation. It was of help to deep understanding rumen animalcule and laid the theoretic foundation for subsequent research.Secondly, the parameters which affected the fermenting experiment have been studied. The biological simulation in this dissertation was the main method, making the parameters should be studied. We found after the experiment that maize stalk granularity was 100 items. The copper and zinc admixed liquor and man-made saliva has been added to rumen fermenting diluent liquor, with man-made saliva twice as the rumen diluent liquor, and it would be helpful to fermenting carried on. After confirming the external parameters, this research mainly focused on optimizing the internal parameters for the optimized combine for fermenting experiment. So that the fermenting production radio of the reverted sugar could be at its most. The regression equation of fermenting temperature, pH measure, time, maize stalk and the production ratio of reverted sugar has been built with general circumrotated experiment of quartic equation with four variables as below:The influencing degree of parameters to fermenting experiment above has been sorted order, after analyzing, from big to small as pH measure, time, maize stalk granularity, and fermenting temperature. Also, the four parameters above had different affection on growing speed of reverted sugar production, with pH measure the most. While the research in the affection of four parameters above to fermenting experiment carried on, this dissertation work also made research in affection of interaction to the experiment production. It has been found that only pH measure and item quantity of stalk powder had interaction, with others having no interaction.The other key point in this research dissertation was to find optimized parameter combine for the most production ratio of reverted sugar. After analyzing, it has been found the production ratio of reverted sugar could reach to 23.74%, at most, with the fermenting temperature 40℃, pH 6.5, granularity of maize stalk 100 items, and the fermenting time 120 hours. Because it is not easy to control the range of parameters in practical industry production, 94 groups of experiment scheme with production ratio above 18.76% have been selected after regression model optimizing.The range of parameters has been confirmed as temperature 37.843～38.162, pH measure 6.614～6.738, time 100.272～106.536 hours, granularity item of maize stalk 100.84～108.52.Producing sugar by simulating internal rumen biological environment would become some new method for sugar. And this method could lay the foundation for sweeping production for its general fermenting qualification and low power wasting.Innovation in this dissertation work are as below:1) The biological growing speed and time has been confirmed by using Photoshop software.2) To find the data & prosperities of rumen in applied industry, by conducting experiment on microorganism of rumen and fermenting, are of applying value to some degree.