Assembly Of Microarrays For Detecting Exogenous Genes In Genetically Modified Plants And Detecting Rice Genome With Xa21 Gene By Microarray Technology

With the number of transgenic plants authorized for release was increased rapidly, food safety and environment risk are being discussed, resulting in many countries and areas have issued GMO labeling regulations for protecting the consumers's authority. Thus, research for the detection of GMOs is necessary in order to verify compliance with labeling requirements. We have developed an array-based method to detect exogenous genes in genetically modified(GM) plants. Microarrays of 54 oligo-nucleotide probes involved 35 exogenous genes of GM plants permitted to grow in the field were assembled.The array was used to detect the exogenous gene xa21 from GM rice DNA. 1 ng of DNA was given a whole-genome amplification with phi29 polymerase followed by fluorescence labeling. Array hybridization showed the positive result.