Genetic Analysis And Fine Mapping Of Pgl3 (t) Mutant In Rice

Chlorophyll is one of the most important pigments for photosynthesis. The content of chlo-rophyll is intimately related to the photosynthesis efficiency and nutrition and bio-outputs of the plants. As one of the most important food supplies and the ideal model plants in the study of de-veloped and molecular biology of the monocotyledon, it not only offer theories and evidence for showing us the mechanism of chlorophyll metabolism, regulation of gene expression and the development of chloroplast, it's also of grate importance in breeding of supper rice.A leaf color mutant was identified from the descendants of an Indica variety Nipponbare which has been dealt with EMS in our laboratory. The mutant displays a pale green leaf during the whole life stage, with low grain set rate. After several generation growed in Hangzhou and Hainan, we found that the characters of the mutant can be geneticed stably. Genetic analysis showed that the mutant is controlled by a single recessive nuclear gene which is not allelic with the known genes. So it's a new gene related to leaf colour. The mutation was temporarily desig-nated as pgl3(t)(pale-green-leaf temporary) since there has reported the gene named pgl2. Chlo-rophyll anlysis stated that the contents of both chlorophyll a and b showed a decrease compared with the wild type Nipponbare in the tillering stage.We used a F2 population generated from the cross between the mutant pgl3(t) and an Indica variety TN1 to map the Pgl3(t) gene. The Pgl3(t) gene was finally located to a 64 kb region be-tween two new designed markers c2 and c3 on the long arm of chromosome 10 after primary, fine mapping. Sequence annotation revealed that there are three ORFs in this 64kb region, all of which had a full length cDNA support. Although we had not confirmed which candidate gene is Pgl3(t), the study would be useful for cloning the Pgl3(t) gene and its function analysis.