Study On Refined Craft About Pupa Bombycis Protein Of Silkworm

Our country is the largest silk big producing country in the world,and Pupa bombycis is regarded as the main by-product of silk industry,it is a kind of natural silkworm's white matter resource with great potentiality.The Pupa bombycis resources of our country are extremely abundant, the leftover bits and pieces of reeling mill are more than 200,000 tons and create favorable condition for comprehensive utilization of Pupa bombycis resources alone every year.The Pupa bombycis protein accounts for 14%-15%of fresh pupa's weight,accounts for 40%-50%which are done pupa's weight,accounts for about 80%of defatted pupa's dry weight.The pupa protein contains 18 kinds of amino acids,among them,the essential amino acid of 8 kinds of human body accounts for 44.1%of all amino acids,and the proportion is suitable,accord with standard is easy to absorb,utilize,a ideal high-quality albumen can offer low-priced raw materials to biochemical pharmacy and edible albumen at the same time than rhymed formula comparatively.So,probe into the Pupa bombycis protein and prepare course and look for Pupa bombycis' albumen especially Pupa bombycis albumen of the high purity to make the craft bestly,can not merely lengthen the industry chain of callus silk trade of our country,increase peasant's income,improve industry's competitiveness,and can be our country medicine,food offer a large number of low-priced proteins,promote the development of relevant trades.This text,through various actual effects of drawing the protein method of systematic comparison,this text,through getting the refined craft to study to Pupa bombycis' albumen,probe into the decolourization of Pupa bombycis' albumen in detail separately,take off the making technological process of the bad smell and high purity Pupa bombycis' albumen,look for out one's own best process conditions,get the following conclusions specifically:1,The degrease craft of the protein of Pupa bombycis is studied,adopted the asking for type to draw the law and soak the formulation to defat Pupa bombycis,it investigate 4 piece factor(method,time, solvents different,it is the firm for liquid than)impact on degrease result:①As regards degrease method,drew the law and superior to soaking the formulation in asking for type.②As regards degrease performance of the solvent,it is the best with propanone that the research institute selects the solvent.③When regarding propanone as solvents,person who ask for draw law,liquid firm than under the 2:1 terms,the extension of time of the reflux has not been influenced to decolorizing,can get the white,Pupa bombycis albumen without bad smell,degrease rate is up to 100%;And ethanols anhydrous under being at termses the same(asking for type draw by law,liquid is the firm than for 2:1),the extension of time of the reflux has not been influenced to decolorizing,has had growth to the degrease result a little.2,Taking off the discredited craft to study of protein of Pupa bombycis(1)Sodium chloric acid once takes off the discredited craft and studies this experiment and chooses temperature,sodium density chloric acid once,horizontal form factor of three factors five of complex reaction time to take off the discredited experiment to Pupa bombycis' protein powder, choose 3 levels finally,carry on the experiment according to the orthogonal form.Obtain the best process conditions for 60℃of temperature,0.8%of density of sodium chloric acid once,take off discredited 8min.(2)Single kind of fibrous form factor analysis takes off discredited research and investigates the defatted wool separately,wheat bran fiber,ginger and fiber,carrot and fiber,Pupa bombycis fiber, konjaku and fiber,apple and fiber,cane sugar fiber,soybean fiber,beet fiber,make six horizontal form factor take off bad smell to test(whether each factor fetch 6 competence 0.5%,1.0%,1.5%, 2.0%,2.5%,3.0%).It isβ- CD in proper order sequentially that five kinds of adsorbents take off the discredited result＞Active charcoal＞α- CD＞Colloidal silica＞active white soil.(3)The compound adsorbent takes off discredited research and combines(2)the experimental result,choose three kinds of better adsorbents to make orthogonal experiment.It is thatβ- CD is 1.5%to obtain the best experiment scheme;The active charcoal is 1.5%;α- CD is 0.5%.(4)Single kind of fibrous form factor analysis takes off discredited research and investigates the defatted wool separately,wheat bran fiber,ginger and fiber,carrot and fiber,Pupa bombycis fiber, konjaku and fiber,apple and fiber,cane sugar fiber,soybean fiber,beet fiber,make six horizontal form factor take off bad smell to test(whether each factor fetch 6 competence 0.5%,1.0%,1.5%, 2.0%,2.5%,3.0%).Every factor is to taking off the main order that the discredited result influence: Defatted wool,ginger and fiber,wheat bran fiber,bagasse fiber,konjaku and fiber,beet fiber,carrot and fiber,Pupa bombycis fiber,the apple and fiber,soybean fiber.(5)Choose defatted wool,ginger and fiber,wheat bran fiber,bagasse fiber,konjaku and fiber, beet fiber six factors to make orthogonal experiment according to(4)the experimental result,and consider the former three reciprocation.Obtain best scheme defatted wool 1.5%,fiber is for 1.5%, fiber is for 0.5%,fiber is for 1.5%,fiber is for 0.5%,fiber is for 0.5%beet bagasse wheat bran konjaku ginger.3,High-quality house Pupa bombycis protein best to draw craft study (1)This experiment adopt cable some deposition methods come on,draw Pupa bombycis albumen,to drawing temperature,time,bath than and pH,etc,more important to extract there aren't factor.(2)Studies have suggested to extract temperature and exert a largest influence on having rate of the Pupa bombycis protein,with extracting the rising of temperature,the higher the having rate of pupa's albumen is,this is because temperature is the higher,the easier to dissolve out pupa's albumen is.(3)The impact on extracting rate of pH takes second place,the best pH extracted of Pupa bombycis' egg is 8.5.In addition,can find out from experiment pupa albumen under optimum pH get rate want,far higher than other value,this because protein molecule until net electric charge zero when the electricity is clicked,static repulsion to reduce molecule,therefore is easy to assemble and precipitate,the solubility is minimum at this moment.(4)It is neither than having a little effect on extracting rate one in bath nor best without being regarded 1:10 as for bath than,greater than or smaller than by bath than,having rate of pupa's albumen is all relatively small.(5)Time to pupa influence that albumen must lead least prominent,with the extension of time, pupa albumen get rate increase big,it means pupa albumen need shorter time can dissolve,come out only.(6)Reply analysis indicating to experimental result of extremely bad law newly according to Duncan,extract Pupa bombycis high-accuracy albumen best to draw terms make up for temperature 75℃,pH 8.5,bath than 1:10,time 2.5h in theory.