Study On Analysis Of Resistance-related Proteins In Rice Against Magnaporthe Grisea By Two-dimensional Gel Electrophoresis

Pyricularia grisea is one of the most serious diseases of rice in the world. To understand the mechanism of the resistance of rice against Magnaporthe grisea, differential proteomic comparison between resistant and susceptible variety before and after Magnaporthe grisea infestation was conducted using two-dimensional (2-D) gel electrophoresis to find the different resistance-related proteins to Magnaporthe grisea. The total proteins induced from four kinds of callus were isolated by TCA/cold acetone precipitation. And these protein samples were separated by means of immobilized pH gradient (IPG) based on two-dimensional gel electrophoresis. After silver staining, the differentially expressed proteins from four kinds of samples were analyzed by using image analysis software (PDQuest). Using the method described above, satisfactory 2DGE maps of total proteins were obtained and the preliminary analysis results were reported. The results showed that before and after Magnaporthe grisea infestation, average numbers of protein spots of resistant variety were 447 and 523, and the corresponding average matching rates were 89% and 87%. The average numbers of protein spots of susceptible variety were 440 and 326, and the corresponding average matching rates were 86% and 85%. The 76 differential protein spots were identified between the two treatments of callus from resistant variety, and the 114 differential protein spots were identified between those from susceptible variety. The differentially expressed proteins can be observed in resistant and susceptible rice to Magnaporthe grisea, which can be used to elucidate the pathogenesis of Pyricularia grisea for further study. Our research results suggested that the changes of protein after Magnaporthe grisea infestation might be the base of resistibility inside the rice.