Analysis Of The Kinematic Parameters In Hyperactivated Spermatozoa And The Gene Expression Characteristics Of Soluble Adenylyl Cyclase

The kinesis parameters incubated in different time and in TALP without BSA of Guinea pig spermatozoa was analysis by CASA.And the expression characteristics of soluble adenylyl cyclase (sAC) were analyzed by our experiments. The expression speciality in tissues and its expression of sAC in testis of mouse at various stages of development were observed by the half-quantitate RT-PCR to study the function of sAC in the process of spermatogenesis and sperm hyperactivation.In the first section, a computer-assisted motion-analysis system was used to describe the kinesis parameters incubated in different times and in TALP without BSA of Guinea pig spermatozoa. The results showed that curvilinear velocity, average path velocity and amplitude of lateral head displacement abruptly increased between 1 and 5 h. However, straight velocity, linearity, straightness and beat cross frequency gradually changed with time. And the sperm motility was not affected by BSA.In the second section, the expression of sAC in eight tissues were analyzed respectively by RT-PCR. The results indicated that sAC was detected in eight tissues. Furthermore, sAC was expressed preferentially in testis and epididymis.In the third section, the expression changes of sAC in different-aged mice were analyzed by RT-PCR. sAC gene expression was developmently regulated with a direct correlation between sAC expression and mouse sexual maturation. The resμlts indicated that the expression of sAC began from 2 week of age, and the expression amount was much low. Then the expression amount increased significantly till 6 weeks of age, from which the expression increased gradually into a stationary phase.