The Preliminary Study Of Chimera In Populus Tomentosa Carr.

A clone in Populus tomentosa Carr. from Ningjin was concluded to be a chimera, which is similar to Chinese white poplar (Populus tomentosa Carr.) such as leaves. But the differences between the chimera and Chinese white poplar was obvious: There were vertical arrises on the parts of its grey-green epidermis as simon poplar (Populus simonii Carr.) and the other parts were smooth as Chinese white poplar. Moreover, the survival ratio of shoot cottage (96.4%) was higher than the Chinese white poplar (38.2%). The seedlings arised from shoot crttage consisted of three types: Chinese white poplar similitudes (88%), simon poplar (2.6%) and chimera (9.4%).1.The peroxidase isozyme zymograph showed that the chimera was similar to Chinese white poplar while not to simon poplar, but the isozyme zymograph of simon poplar seedling arised from chimera was like simon poplar. This indicated the chimera was related to Chinese white poplar and simon poplar.2.The relationships between the rooting of the cutting of the chimera and the normal of Chinese white poplar (populus tomentosa Carr.) and the activities of peroxidase, indoleacetate oxidase, the superoxide dismutase and the content of dissoluble protein ware analyzed.3.In the stages of cutting rooting of the chimera of P. tomentosa, the activity of peroxidase increased after cutting and got to the first peak at the 9th day, and followed to decrease and got to minimum at the 18th day, the second peak appeared at the 21st day. The change curve found in normal P. tomentosa was reverse to the chimera at first 9 days and from the 15th to 21st days. The indoleacetate oxidase activity was similar to peroxidase. The activity of superoxide dismutase decreased after cutting and got to the minimum at the 9th day in the stages of cutting rooting of the chimera of P. tomentosa, then increased. The activity of superoxide dismutase of P. tomentosa increased at the beginning of cutting and got to the peak at the 9th day, then decreased. The activity of dissoluble protein between the chimera of P. tomentosa and normal P. tomentosa was contrary from the 15th day to the 27th day, and the chimera of P. tomentosa was higher than the normal in all.As a conclusion, the chimera rooting phase was related to the activities of peroxidase and indoleacetate oxidase as well as superoxide dismutase and dissoluble protein. Normal Chinese white poplar did not easily root because it could not satisfy the relative conditions.