Genetic Diversity And Relationship Of Tomato Based On AFLP Marker

Tomato (Lycopersicon esculentum Mill.) is one important kind of popular vegetable, it is one principal kind of cultural vegetable in china. The genetic diversity and relationship of 47 cultivars of tomato resources introduced from Russia, Japan, Italy and china were evaluated using morphological marker and AFLP molecular markers in this study. The main results as followed:(1) Analysis of morphological marker for 47 cultivars of tomato resources was significantly to carry out. Morphological diversity among 47 cultivars of tomato resources was discussed by 14 characteristics. 47 cultivars of tomato resources were divided into 3 groups according 14 characteristics with Euclidean distance by UPGMA. 3 cultivars introduced from Russia belonged to Group 1; 39 cultivars belonged to Group 2; 5 cultivars belonged to Group 3. 14 characteristics were analyzed using R clustering and principal component analysis.(2) The genetic diversity and relationship of 47 cultivars of tomato were evaluated using AFLP molecular markers in this study. The results showed that a total of 575 informative AFLP fragments were generated with 10 EcoR?-MseΙprimer combinations, of which 384 ones were polymorphic and the polymorphism rate was 66.78%. The average number of DNA bands per primer pair was 57.5. At a similarity coefficient of 0.80, 47 cultivars of tomato resources were divided into 3 groups according 14 characteristics with Euclidean distance by UPGMA dendrogram. N-3 and M-16 introduced from Russia belonged to Group 1; 44 cultivars of tomato belonged to Group 2, At a similarity coefficient of 0.82, the 44 cultivars of tomato could be divided into two subsets, the 19 cultivars of tomato resources belonged to subset 1, 25 cultivars of tomato resources belonged to subset 2; Jianhuang belonged to Group 3.(3) Most classification by morphological marker and DNA marker are same, but there is a little difference between them. The objective of this study was to provide scientific bases for establishment of gene pool, selection and breeding of eminent cultivars, protection and usage of germplasm resources and their relationship in tomato, DNA fingerprints of tomato could also be used for marker-assisted selection. Therefore, the results would exert important effects on genetic improvement of tomato at the molecular level.