The Roles Of Callose In Soybean Against Soybean Mosaic Viruses

In order to explain the possible physiology mechanism of soybean against soybean mosaic viruses (SMV) infecting, these experiments were caeeied out. In the experiment, Soybean plant [Glycine max (L) Merr.] cv. Jidou 7 and strain N3 of SMV as incompatible combination, cv. Jidou 7 and strain SC-8 of SMV as compatible combination were tested. The roles of callose in soybean against SMV transport between mesophyll cells were studied using aniline blue fluorescent dye staining, callose immungold, sphincter tannic acid staining and activity measurement ofβ-1,3-glucanase. The result of aniline blue fluorescent labeling showed that: there were lots of callose fluorescent in and outside the leision in incompatible combination, but no callose fluorescent outside the lesion in compatible combination. The optical level proof indicated that the callose involved in the resistance course of soybean against SMV infection process. The results of immunogold labelling indicated that: there were gold particle deposited in plasmodesmata in or outside the lesion in incompatible combination, but no gold particle deposited in plasmodesmata in or outside the lesion. The results from observation by the immunogold labelling indicated that the callose involved in the resistance course of SMV infection. To further explore the roles of callose in soybean against SMV infection, tannic acid staining of sphincter were carried out, there was sphincter deposite at the neck of plasmodesmata prior to local lesions formation and after local lesions formation in incompatible combination, but no sphincter deposite at the neck of plasmodesmata all the time in compatible combination. Through the activity measurement ofβ-1,3-glucanase showed that: there was a low level ofβ-1,3-glucanase activity prior to local lesions formation and after local lesions formation in incompatible combination, but there was a high level ofβ-1,3-glucanase activity prior to lesions formation in compatible combination. The biochemistry level proof indicated that the callose involved in the resistance of soybean SMV infection process.Through the above results, it suggested that plasmodesmata are pathways for intercellular movement of virus particles and spread of virus may be prevented by callose deposition in plasmodesmata.