The Hybrids Of Intergeneric Hybridization Between Apricot And Almond And Their Identification By SSR

The experiment material is apricot and almond in this paper,passed TTC method to carried on examination the agnate pollen of vitality before pollination;after the investigation fruit set percentage,using of DPSv3.01 analyzed the date;the pollen tube behavior and bourgeon were observed by means of flurescence microscopy;the orthogonal design was used to optimize SSR amplification system of apricot hybrids in five factors at four levels respectively;making use of the new SSR-PCR system analyse the 50s hybrids. The main results were as followed:1.The TTC method was use to observe the agnate pollen,the pollen has energy to bourgeon and satisfied pollination requirements,but this method have some defect that was not to show the power of bourgeon;the result of investigation fruit set percentage was analyzed by DPSv3.01,between the cross combination were very difference(α=0.05).2.The pollen tube behavior in the style following the hybridization between apricot and almond were observed by means of flurescence microscopy.The results indicated that the pollen of almond can germinated on the apricot and pollen tube can reach the basal part of the style and finally arrive at the ovule in ovary.Meanwhile,the result also showed that the number of pollen tube in the middle parts has decreased,just a few pollen tube can reach the basal part of the style and finally arrive at the ovule in ovary,several abnormalities of pollen tubes were observed including pollen tubes with swollen or pointed parts,or pointed tips and snap and superposition in the middle parts,but there hasn't lots of callse depositing at irregular distances.3.The orthogonal design was used to optimize SSR amplification system of apricot hybrids in five factors at four levels respectively.A suitable SSR reaction system was established by range method,namely 20μL reaction system containing 1×buffer,2.0mM/L Mg²⁺,0.25mM/L dNTPs,0.25μM/L Primer,60ng/20μl DNA templet and 0.05 U/μl Taq polymerase.4.In the new SSR-PCR system,there have got 13 primer pairs from 32 primer pairs that they showed strong signal and high polymorphism.The parents and hybrids etc 55 samples analysed by Using 13 primer pairs,the experiment results show that the average identification rate of 13 primer pairs came to 69.69%,the identification rate of 9 primer pairs(69.23%)came to 70%among them,the identification rate of primer pair 13 and 30 were highest(100%),the primerpair 24 was lowest(10%).This shows,the SSR of selected primers have strong signal and high polymorphism,they can identify the 50 hybrids.5.UPGMA analysis of bands of amplification products applying NTSYSpc 2.02a software,cluster analysis based on genetic similarity coefficient that the GSC of 55 experiment materials were from 0.65 to 1.00(avrage 0.83),the GSC of 52 materials from 0.74 to 1.00 among them(94.5%).The dendrogram shoe that the 55 materials were divided into five class at the line L₁,the agnate No.4 was a class and matriline longwangmao was a class,the other three agnate and hybrids were regular cluster,only agnate No.1 and P6-N-4,P6-N-7,P6-N-8 etc.14 hybrids were cluster,show partial male type.