Physiological Detection Of Zoysia Japonica Transformed DREB1A Gene And Molecular Detection And Cold Tolerance Of Offspring

In before research job, the laboratory adopt the gene gun to having obtained transgenic plants of zoysia japonica transformed DREB1A gene which having security. In this research,We studied phenotype and resistance physiology. Transgenic plants were treated with drought, salt and cold stress, and then the Relative leakage electrolytes, concentration of MDA, PRO, SOD and POD enzymes activity are measured. The results showed that, transgenic plants have higher drought, salt and cold resistance than that of the control. Preparation by screening take place has the transgenic plant having definitely resisted contrary capability being 22. Force inbreeding by the bag carrying out cover, the molecule having been in progress to self relationship gains descendant identifies that, PCR and Southern blot indicated the DREB1A gene had integrated into the genome and inherited to the offspring, and having ascertained the 10 transgenic plants department in descendant. transgenic plant is that the cold resistance carrying out seedling stages is analytical to this 10, the strong plant sifting out 4 seedling stage winter resistance ultimately is. Close offspring of contrast resisting goes against nature , the gene and the transgenic plant showing certain resistance are external source the breeding produces the parental generation 4 having stabilized inheritance. The safety being that self own manages office as a result of having gained PRC forest genetically modified organism safety appraises revolution of DREB1A gene plant that the experiment gains , self own enters the centre experiment now.