| Trichoderma reesei,belonging to Deuteromycotina Hyphomycetales,is the anamorph of Hypocrea jecorian.At present,main researches focus on molecular mechanisms of enzyme production including its gene cloning and sequencing,excessive expression of recombinant protein and genetic modification of high enzyme production strains.However, studies on the molecular and cell biology,especially,on cell autophagy in T.reesei have not been reported yet.Autophagy is a common,functionally and evolutionarily preserved process that degrades and recycles old proteins and organelles in all eukaryotic cells.Autophagy has been presumed to be involved in cellular architectural changes,presumably via its role in organelle and protein turnover.The purpose of this study is to find out the main function of TrATG5 gene in T.reesei, and to clarify the roles of autophagy in T.reesei during differentiation and development.In this study,we have isolated one autophagy-related gene,TrATG5,from the genomic bank of T.reesei.Then we successful constructed a deletion vector and sought a way to knock-out the gene by means of Agrobacterium tumefaciens-mediated transformation.As a result,ΔTrATG5 mutants were obtained,in which TrATG5 was deleted in the wildtype T. reesei QM9414.ΔTrATG5 mutants,show defective phenotypes,such as sparse mycelium growing on PDA medium plates,lower mycelium growth rate.The clony appear on yellow owing to its dramatically decreasing green conidiation.We also found its mycelium have less branches than the wildtype.ΔTrATG5 were confronted with Rhizoctonia solani and then were found that its mycelium can wrap R.solani.It was reported that T.reesei fail to utilize sucrose.However,data of our reductive sugar measures show theΔTrATG5 succeed in the utilization of sucrose.In summary,TrATG5 gene plays a significant role in autophagy of T.reesei,which is responsible for fungal morphogenesis and ensures the turnover of organic matter necessary for spore formation.
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