Formulation Of Bio-Control Actinomyces Strain 153

The solid fermentation method of actinomycetes strain 153 isolated from soil which having strong biocontrol capability was studied. The conditions of solid fermentation were optimized, the technique and additive for formulation were determined.Actinomyces strain 153 can produce abundant spores on several farm produces (i.e. bran, corn flour, millet flour or soybean flour etc.), only need minimal element. The optimal range of temperature at 25 to 28℃and the optimal humidity was 800mL per kilogram dry substrate, additional, the optimal quantity range of inoculums (109cfu/mL) at 4% to 8%. The quantity that a bottle holds was important for solid fermentation of strain 153. So that the quantity of air should be controlled when ferment strain 153.Strain 153 was formulated as a live-cell powder by mixing propagules with diatomite, kaolin or non-cultivated loess. With different additive, stored time of the formulation was influenced obviously. And non-cultivated loess was better than the others. With this additive, the biocontrol agent could be stored more than 10 weeks at 35℃, and the biomass of this formulation surviving 71.57% that had been stored at 4℃for 10 weeks. That probably because actinomyces strain 153 was isolated from soil, so it adapts the conditions of loess.The growth and development of eggplant could be promoted obviously by live-cell formulation of strain 153. Especially, the amplitude of green plant weight attained 98.47%, amplitude of dry weight of stem and dry weight of radix attained 74.86 and 82.64%.The eggplant Verticillium wilt could be controlled effectively by live-cell formulation of strain 153 in greenhouse. The rate of control of eggplant Verticillium wilt with formulation mixed non-cultivated loess reached 82.44%. The control rates of the other two formulations were also over 70%. Thus, actinomyces strain 153 can be formulated as a live-cell powder to control some soil-borne diseases and to promote growth of plant.