| Lily is a world famous bulbous-flower and cut-flower, take very important part in world flower market. As a kind of ethylene lead decay flowers, flower decay rapidly while blooming with ethylene's release. Liliaceous view and admire life will be prolonged, and the value of lily be raised if we can effectively control the release of ethylene. We did the genetic transformation of ACO antisense gene into lily's bulb and leafstalk by Agrobacterium tumfaciens-mediated method. And exogenous genes were identified by GUS gene coloration, PCR, ladderlike purification-based PCR method and southern blot analysis. Besides, we did some research on transplant of tissue culture seeding.1. 165 hygromycin-resistant plantlets were obtained through Agrobacterium tumefaciens-mediated transformation of ACO antisense gene into lilium oriental'sorbonne'2. Ameliorated the protocol of Agrobacterium tumefaciens-mediated transformation established by our lab. 15.76% genetic transformation rate was obtained according to the procedures: the explants without pre-cultured, immersed into bacterial suspension (OD600=0.8) vacuum degassed for 1min, slowly waggled for 30 min, and then co-cultivated for 7d.3. Transformation plants were identified by histochemical GUS gene detection, PCR, ladderlike purification-based PCR method,southern blot and PCR-southern blot analysis. 26 positive plants were got out of 85 plants by PCR analysis, 1 positive plant was obtained by PCR-Southern.4. According to studies on the effect of the selection time on hygB added medium on lilium exogenous genes transformed by Agrobacterium tumefaciens-mediated method, the effects of persist selection to transformation plants was ascertained.5. A Transplant system was initiated from lilium oriental'sorbonne'. The medium Perlite/peat 1/1 was identified as the best transplant medium. And we did some elementary research on different exogenous phytohormone's effect on the transplant seeding. The suitable consistency is IBA 50mg/L; NAA 50mg/L; GA3 6mg/L.
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