| With the development of modern agriculture, the technology of pollination by utilizing pollinating insects is becoming more and more important. In this paper, we studied the different activity patterns in pollination including the time and the temperature of out-nest,the visiting time,inteval time,frequence and the weight of pollen between Bombus hypocrita and Apis mellifera. We also studied the preference to purple flowers of Bombus hypocrita under laboratory simulation. The results were as follows:The out-nest time of Bombus hypocrita is fixed at 8:15～8:30 while the out-nest time of Apis mellifera depends of the weather. It is usually at 9: 30～10: 00 on fine days and 9:30～11:30 on cloudy days. Meanwhile, the out-nest temperature and humidity are 7.19±0.07℃and 86.77±0.26( %) of Bombus hypocrita and are 14.30±0.04℃and 87.46±0.13( %)of Apis mellifera, respectively. The T-test results of out-nest time under different weather were not significantly different for Bombus hypocrita ( t=-2.479, P> 0.05) and were extremely significantly different for Apis mellifera ( t=-14.186,P< 0.01). At the same time, the T-test results of out-nest humidity under different weather were significantly different for Bombus hypocrita( t=-2.405, P< 0.05) and were extremely significantly different for Apis mellifera( t=-14.771, P< 0.01) .The day-time activity regularity of Bombus hypocrita is similar to that of Apis mellifera under the same weather (sunny) . For Bombus hypocrita, there are two crest-time in a day, one is at 10: 00~11: 00 and the other is at 13: 00~14: 00. The number of out-nest bumblebees during the first crest-time is higher than the number during the second crest-time. For Apis mellifera, there is only one crest-time in one day which is at 11: 00~12: 00. By comparing the day-time activity regularity of Apis mellifera under different weather, we conclude that the out-nest time is at 12:00~13:00 on cloudy days which is one hour later than that on fine days. The total number of out-nest bees on fine days is about as five times as the number on cloudy days. By analyzing the dependablity between the out-nest number and three environmental factors, we conclude that temperature is the main factor which effect the out-nest number of bumblebees and that the effect of intensity of illumination is not significant and that the humidity and the out-nest number are negatively correlative. However, for honeybees, the three environmental factors'effect is significant. Temperature and intensity of illumination have positive correlation with the our-nest number while humidity has negative correlation with the out-nest number.The results of visiting time,inteval time and frequence are different between Bombus hypocrita and Apis mellifera under distinct temperature. The visiting time and inteval time of bumblebees are shorter but the frequence are higher than that of honeybees. Additionally, the results'variability of visiting time of differently morphological flowers is extremely significant for Apis mellifera.The weight of the pollen which collected by bumblebees is bigger than which gathered by honeybees and the sedimentation coefficient of the former is bigger than that of the later. All T-test results are extremely significantly different.In different mock-up test groups, the preference to purple flowers of Bombus hypocrita are different. The D-value is stable and not significantly different when we change the number of colors. It reveals the specificity to purple flowers of Bombus hypocrita when facing varicolored flowers. The D-value changes significantly when we alter the floral design. Size and shape have more effect on visiting to purple flowers than odor does. The D-value is significantly different when the characters adding to flowers are different.
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