Cloning And Sequence Analysis Of L Gene Polymerase Activity Module Of Rabies Virus Isolates From Guangxi

In this study the cDNA fragments containing the L gene polymerase activity module of rabies virus isolates from Guangxi were cloned and sequenced. The nucleotide sequence of L gene activity module and their deduced amino acid sequence were compared with those of other rabies virus strains and rabies-related virus strains published previously.Comparison of the nucleotide sequence and deduced amino acid sequences of polymerase activity module of rabies virus isolates from Guangxi with that of other fixed rabies virus strains and rabies-related virus strains. The nucleotide homologies and deduced amino acid homologies between isolates from Guangxi and fixed strains were 84.8% -87.5 % and 94.5 % -99 % respectively, while Guangxi isolates and rabies-related strains were 75.5%-79.2% and 93.5%-97%, respectively. The nucleotide homologies and deduced amino acid homologies between Guangxi isolates were 88.7%-99.8% and 96.5 %-100% respectively.According to the nucleotide sequence of L gene polymerase activity module, we analyzed the homology identity of the Guangxi isolates. Three group were divided. Group I include strain GX260, GXWX, GX195, GX014, GX08, GX09, GXHX, GXLA, GXSL and GX091. Group II include strain GX074, GXPX, GX01, GXBM, GX219 and GX304. Strain GXN119 belongs to Group III.Compared the amino acid mutation of the L gene polymerase activity module, The amino acid specific mutation of Guangxi strains were position 660, 754 and 771, on the amino acid position 754 of L gene, Guangxi strains all were arginine, while serine, leucine or histidine were found at the same position of other rabies virus strains and rabies-related virus strains. On the amino acid position 660 of L gene, GX01, GX074, GX219, GX304, GXPX and GXBM strains were all Valine; and on the amino acid position 771 of L gene, GX08, GX09, GX014, GX091, GX195, GX260, GXHX, GXLA, GXSL and GXWX strains were all alanine.Compared the L gene polymerase activity module, the four motif were quite conserved, Motif A is the most conserved domain in the four motifs, the Motif B, C, and D, showed several amino acid change, the core sequence CDNQ of Motif C in all strains keep conserved.