Comparative Genomic Analysis Of Autopahgy Related Genes And PBI2 Cloning And Function Analysis

Autophagy is a process for degradation of long-lived protein and organelles.This process is highly conserved in eukaryotes.Cytoplasm to vacuole targeting(Cvt)is a constitutive biosynthetic pathway required for transport of aminopeptidaseâ… (Apel).The two pathway share many autophagy related proteins in Saccharomyces cerevisiae.Using published Atg protein sequences,we have identified many homologs in 5 plant pathogenic fungi genome databases.To further understand evolution condition of this highly conserved process,we analyzed conserved protein structure of these homologs.This analysis suggests that Atg17 was disappeared step by step in evolution from Saccharomyces cerevisiae to higher eukaryotes..Two different strategies were adopted for evolution some components of this system,one of them is through fusion or fission of protein architectures.Another one is through increasing copy numbers of genes.Magnaporthe grisea(M.grisea)is an important ascomycete that causes rice blast.Studying of the disease-caused basis of M.grisea is not only beneficial for rice blast control,but also serve as a model for revealing other fungal pathogen-plant interactions.Fusion between vacuole and autophagsome is a downstream process of autophagy.Vacuole is considered to be a lytic compartments.Many processes of material degradation in cell occurs in vacuole lumen,which contribute to protein turnover and energy transferring,lipid degradation in vacuole is very important in maturing appressoria.Vacuole of Saccharomyces cerevisiae projects a stream of vesicles and membranous tubules into the bud where they fuse and establish the daughter vacuole. So,Vacuole formation in daughter cells through fusion of vesicles inheritance from mother cell is very important in maturing appressoria and effecting its infection ability.