Study On The Enzyme Activity And Histology Of Intestine In Pingxiang Red-transparent Crucian Carp, Carassius Auratus

Anatomy,histology,immunohistochemistry of intestine and activities of digestive enzyme of Pingxiang red-transparent crucian carp were studied by the methods of morphology and enzyme kinetics in this paper.The results were as follows:Pingxiang red-transparent crucian carp had no stomach,esophagus was followed by intestine.The intestine travels as the route of "S" in the body cavity.The intestine includes foregut,midgut and postgut.The beginning of the intestine expands, and the diameter of gut was getting thin from foregut to postgut.The regression equation of the weight(Y)and the body length(X)was Y=0.0309X^3.0296(R²=0.9531). The ratio of gut length to body length was 3.73.The wall of intestine consists of mucous membrane,submucosa,tunica muscularis and serosa membrane.Tunica muscularis was divided into inner circular muscularis and outer muscularis.The number and the height of tunica mucosa folds decreases gradually from the anterior intestion to the posterior(P＜0.01);but the number of goblet cell changes from few to many.The mucous cells in the intestine of Pingxiang red-transparent crucian carp can be classified into 4 types:typeâ… ,typeâ…¡,typeâ…¢,typeâ…£.The foregut was rich in typeâ… cells,the morphology of the foregut mucous cells were round or goblet;the midgut rich in typeâ…£cells,the morphology like that of the foregut;the postgut rich in typeâ…¢cells,the morphology were main tadpole alike.The morphology of Gastrin cells and Somatostatin cells vary in shape,such as club,round,ellipse,shuttle, pyramid and so on.Gastrin cells and Somatostatin cells occured in the whole intestine and distributed decreases gradually from the anterior intestine to the posterior one. The results on the activities of protease,lipase and amylase in Pingxiang red-transparent crucian carp showed that the activities of protease in the hepatopancreas was significantly lower than that in the intestine(P＜0.01),which in the foregut was much significantly higher than that in the postgut(P＜0.01),and significantly higher than that in the midgut(P＜0.05).There was no significant difference between the midgut and the postgut(P＞0.05).The order of protease activities in the intestine was foregut＞midgut＞postgut.There were no significant differences in the lipase activities among any part of the intestine and the hepatopancreas(P＞0.05).The order of lipase activities was midgut＞hepatopancreas＞foregut＞postgut.The amylase activities in the hepatopancreas was significantly higher than those in the intestine(P＜0.05),and much significantly higher than that in the midgut and in the postgut(P＜0.01),and there was no significant differences among the different part of intestine(P＞0.05).The order of amylase activities was hepatopancreas＞foregut＞postgut＞midgut.