| As the second largest tropic flower, Anthurium is popular for ornamental charm, but existing varieties can not meet the desires of people. Therefore, scientists are trying to change the flower color using genetically modified methods to improve its commercial value.The major objective of this study was to transfer para-carotene biosynthesis enzyme gene (LycB) into Anthurium accumulating uperfluous para-carotene in their leaves, consequently altering their color. The major results of this study were introduced separately as following:(1) Tissue culture system for Anthurium was optimized. First, effects of cultural methods (liquid culture and solid culture) on Anthurium andraeanum callus formation are compared. Results showed that liquid culture was prior to solid culture effectively, and approximately 3～4 times. Second, effects of four factors (NAA,active carbon,lactalbumin hydrolysate,banana mud) on strengthening Anthurium were analyzed and the improved protocol was obtained.(2) An Agrobacterium tumefaciens-mediated transformation protocol was established for Anthurium. Effects of factors such as Agrobacterium concentration,infection time,co-culture time,concentrations of AS,ultrasonic treatment on T-DNA transfer were discussed in detail using callus as inoculation material, then these factors were optimized. The results showed: Agrobacterium tumefaciens-mediated transformation was affected in a certain extent by these factors; in some degree, increasing their levels would improve the efficiency of frequency of resistant callus, but results were not good beyond this range; Optimized transformation was performed by inoculation of callus with Agrobacterium. By infection according to this optimized T-DNA transformation protocol, we obtained transformed plants after PCR and Southern verification.
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