Isolation And Chinensis Promoting Effect Of The Saline-Alkali Tolerance Phosphate Solubilizing Bacteria In Alkaline Soil

Soil saline-alkalifying is becoming more and more severe because of the unsuitable irrigation, eatra-using of fertilizers and vegetation destroying. People have paid more attention to the soil improvement and remediation, including Engineering, chemical and biological measures because of the expansion of population and the reduction of farmland. The construction effect of short-term was good but the input is great. Chemical measures can take effect in the short time with the high cost and side effects.They realized that it is more economical and effective to screen and identify enduring alkali variety than to utilize engineering measures in order to improve saline and alkaline land. So, people like to harness saline and alkaline land with biological treatment. The result of research indicate that in order to consolidate and improve treatment effect and defence the returntion of salts, agricultural measures are necessary, especially the improvement of soil fertility. Therefore, this study want to screen saline-alkali tolerance phosphate solubilizing bacteria used to promote plant growth, in order to ecologically restorat salineSongNen alkaline-saline plain was selected as research objective at present paper based on the view of eco-engineering and ecological rehabilitation of alkaline-saline land. Two strains which posseses strong phosphate-solubilizing capability were selected from alkaline-saline land. Strains of C111 and C141 were identified as Acinetobacter-calcoaceticus (C111) and Candida-parapsilosis (C141) through their physiological characteristics, morphology of colonies and individual, and MIDI analysis.The applicability to environmental factors, were thoroughly studied by determination of the phosphate-solubilizing capability of C111 and C141. Results showed that two strains can tolerate relatively high osmotic pressure and alkalinity and they could keep high phosphate-solubilizing capability in the condition of high concentration Ca2+ .The C141 had better Adaptability than C111, in the respect of carbon utilization. Phosphate solubilizing bacteria could dissolve phosphate and the process was complex. The results indicated that the phosphate solubilizing bacteria might excrete protons and organic compounds and they could solubilize insoluble phosphate through both the way of acidilation, anion-exchange and chelation.Medium was collected at various times after inoculation and centrifuged and the supernatant was measured for phosphate, in order to understand the applicability to alkaline soil. The experimental results presented here show that the microorganisms were able to increase the availability of P of the alkaline soil. It suggests that PSMs could be effective in alkaline soils.Taking the typical plant of Leymus chinensis as target research objective, the effects of inoculating C111 and C141 into laboratorial potted plant were investigated. After inoculation, the heights of Leymus chinensis were higher than than the control buring the whole experiment. The available phosphorous of rhizomes was also determined and the results implied the changes of phosphorous might be owing to the inoculation of C111 and C141 and this might be one of the reasons resulting in the growth of Leymus chinensis. Saline-alkali tolerance PSBs can not only adapt to alkaline-saline soil environment, but also improve the available Phosphorous and change the soil quality.