Produce Trangenic Chicken By Direct Injection EGFP To Testis And Ovaries In Vivo

In recent years, with transgenic technology continues to mature, and the chicken with a short interval from generation to generation, high performance, and the gene can be expressed in the eggs. Researchers gradually be attracted to develop in the poultry, the paper used fat of parcels of green fluorescent protein, through the surgery, injecting liposome-DNA into young cock's testes and hen's ovaries, group accordance to the experimental design After transfection, inseminate artificial, then fluorescence detect and PCR test the different periods, different day's embryos the results of the following areas:1 Injecting transfection staining solutiong to Testicular by multidrop in vivo, and then the rooster manual mining sperm and semen through the extraction of DNA for, and then detect under the microscope, the result appears fluorescence, then the corresponding group in hens artificial insemination, the collection of different-day-old chick embryos for the detection, Transfection rate of pIRES-EGFP in offspring at different periods after operation is 66.67% (40/60) average, from different organizations embryo PCR analysis, the positive rate is 60 to 70 percent, this show multi-point injection pIRES-EGFP is a viable method of producing transgenic animal.2 Injecting transfection staining solutiong to ovarian by multidrop in vivo,,then test the result by three-step method after 48 hours and found that the fluorescence appeared weakly.After that choose the hen who favorable of postoperative recovery to artificial fertilization and the sperma come from the cock which is not operative.Testing the embryonated egg of differential day and founding that the express of green fluorescnt protein in the offspring at different times is 45%,the result of chicking the different tissues of embryonated egg by PCR is 40-60%.3 Injecting the third group the cock and hen at the same time, the acquisition of the rooster surgery done on the transfer of semen over the hen to artificial insemination, and then on to the embryo The same operation, Transfection rate of pIRES-EGFP in offspring at different periods after operation is 66.67% (40/60) average, from different organizations embryo PCR analysis, the positive rate is 60 to 70 percent.4 The result of comparing three groups showed that : northern the expressing rate of green fluorescent protein of offsping nor masccline rate of embryonated egg in group 3 is more then group 1 and group 2.From the result we can obtain it a new method to product transgenic chickens approach the testes and ovaries in a direct injection of green fluorescnt protein genes. Treated the hen and cock at the same time is better then treated only one of them in one time. it a new breakthrough in transgenic method. Transgenic research in poultry on the application of new models for the use of transgenic chickens as bioreactors to establish a technology platform, And seeking a new mehtod which can easy to operate the availability of transgenic chickens in large quantities and Comprehensive genetic information and the embryo was not to cause damage,...