Growth Promotion And Prophylaxis Effects Of Mixed Inoculation With Fungi And Actinomycetes Against Phytophthora Capsici On Pepper

In this study, Trichoderma, Chaetomium and actinomycetes isolated and selected in our lab to antagonize pepper Phytophthora blight were as test strains. We studied on the growth promotion and prophylaxis effects of mixed inoculation with fungi and actinomycetes against Phytophthora Capsici on pepper, explored effect of the multi-inoculums with the antagonistic microbial. The results showed that:1. According to Morphological characters and culture condition, fungi M1and M2 are identified as Trichoderma harzlanum and Trichoderma aureoviride.2. When inoculated without pathogens, antagonistic microbial inoculation could control capsicum Phytophthora blight. When inoculated with pathogens, except some individual combination, antagonistic microbial inoculation could control capsicum Phytophthora blight, relative control effect was 50 %3. When inoculated with antagonistic microbial, effects of promoting pepper growth and production were significant. Based on pot and seedbed experiment, the increasing amplitude of root weight was 70.7 % ~ 245. 5 % and 63.0 % ~ 304.4 %, the increasing amplitude of total biomass was 34.5 % ~ 101.3 % and 171.2 % ~ 333.8 %. After transplanting, antagonistic microbial's inoculation could still promote the growth of pwpper, the increasing amplitude of production was 8.9 % ~ 152.0 %. Best combinations were Act2 with M1 and M2, Act8 with M1.4. Antagonistic microbial inoculation could have significant effect on PPO activity of pepper. Based on seedling stage experiment, antagonistic microbial inoculation could reduce PPO activity of potted pepper root, increase PPO activity of leaf, the reducing and increasing amplitude of PPO activity was 0.4 % ~ 40.2 % and 4.9 % ~ 30.6 %. After inoculated with P3, the increasing amplitude was higher than inoculated without P3 treatment, value was 12.0 % ~105.0 %. Based on mature stage experiment, antagonistic microbial inoculation could increase PPO activity of potted pepper root, reduce PPO activity of leaf, the increasing and reducing amplitude of PPO activity was 5.3 % ~ 20.7 % and 14.2 % ~ 32.0 %. Effect of multi-inoculums with antagonistic microbial and inoculated with P3 was much more significant, value was 0.1 ~ 0.6. 5. Associative inoculation had significant effect on pepper root zone soil micro flora. Associative inoculation of biocontrol fungi with actinomycetes could not promote the colonization of actinomycetes in pepper root, could promote the colonization of actinomycetes on the surface of pepper root and had significant effect on colonization of M2, the increasing amplitude was 209.1 % ~ 672.7 %, only M1 could help the colonization of Act1 on pimiento root, the increasing amplitude was 120 %. Associative inoculation in potted peper could significantly increased the quantity of the bacteria and actinomycetes, The microbial flora of pepper root zone soil changed markedly, it changed to"bacteria type".6. Through orthogonal L9(34) experiment to research the composition of solid state fermentation medium of strain M1 and M2 growth and spore production, on the optimal medium, spore production of strain M1 and M2 was 6.7×109 and 10.6×109 CFU/gThis study provides scientific basis for researching on multi-inoculums with antagonize pepper Phytophthora blight. It also has theoretical significance and application value for controlling pepper Phytophthora blight.