Study On Fermentation Condition Of Trichoderma T115D And Biocontrol Of Trichoderma T115D Against Phytophthora Root Rot Of Soybean

There were Experimental purposes that Trichoderma T115D was applied. The intention of this experiment was to research the fermentation condition of Trichoderma T-115 D, antibiotic activity of Phytophthora sojae hyphae to fermentation filtrate, study on biocontrol mechanism of T115D and Biocontrol effects experiment of Trichoderma T115D fermentation against Phytophthora root rot. Experimental can Provide theoretical basis that development and utilization of Trichoderma Biological pesticides.Through study on fermentation conditions for Trichoderma T115D, the results showed PD is the most suitable medium that Trichoderma T115D produce metabolites in different medium, GPF is second.When Trichoderma T115D is cultivated in PD, spores was easy to produce when pH value was 7.0～8.0, temperature was 30℃, rotate speed was 200～250 r·min-1; hyphae was easy to grow when pH value was 4.0～6.0, temperature was 25～30℃, rotate speed was 150～250 r·min-1; Trichoderma T115D metabolites was easy to produce when pH value was 5.0～6.0, temperature was 25～30℃, rotate speed was 150～200 r·min-1.When Trichoderma T115D is cultivated in GPF, spores was easy to produce when carbon source was Sorbose, introgen source was Potassium nitrate and Ammonium sulfate and Ammonium tartrate, pH value was 8.0～9.0, temperature was 35℃, rotate speed was 250r·min-1; hyphae was easy to grow when arbon source was mannose, introgen source was Yeast extract and Ammonium tartrate , pH value was 4.0～5.0, temperature was 25～30℃, rotate speed was 250r·min-1; Trichoderma T115D metabolites was easy to produce when carbon source was Glucose, introgen source was Ammonium tartrate, pH value was 4.0～7.0, temperature was 20～25℃, rotate speed was 200～250r·min-1. After Trichoderma T115D fermentation filtrate treated Phytophthora sojae, the results showed that Trichoderma T115D metabolites can inhibit growth of Phytophthora sojae hyphae strongly, the sporangia formation and bourgeon of spores. Trichoderma T115D metabolites can change cell membrane penetrabilit of Phytophthora soja. When concentratio and time was increased, it could increase the cell membrane permeability, In other words, injuryof cell membrane was Increase. Induction of systemic resistance was researched by measuring the enzymes activities of PAL, POD, SOD, CAT, and PPO after inoculation of Trichoderma T115D Fermentation and Phytophthora sojae. Result indicated that four enzymes activities was increased after soybean miller was inoculated Trichoderma T115D Fermentation and Phytophthora sojae. When soybean miller was inoculated Phytophthora sojae only, peak value of enzymes activities came early, however other treatments came later and peak value of enzymes activities was more high. In different treatments, Induced effects that soybean miller was inoculated Trichoderma T115D fermentation of include spores in advance and was inoculated Phytophthora sojae in the next day was best.Biocontrol effects of Phytophthora root rot of soybean caused by Phytophthora sojae with Trichoderma were studied under greenhouse condition. An experiment was preformed to evaluate the effects of different methods of inculation, different time of inculation and different measure of inculation. Results showed that different treatments can alleviate Phytophthora root rot of soybean.When we used spray inculation, biocontrol effects that soybean miller was inoculated Trichoderma T115D fermentation in advance was better than biocontrol effects that soybean miller was inoculated Trichoderma T115D fermentation and Phytophthora sojae in the same time. Biocontrol effects that soybean miller was inoculated Trichoderma T115D fermentation of include spores in advance and was inoculated Phytophthora sojae in the next day was best, the Biocontrol effects value is 73.3%. When we used puring root inculation, biocontrol effects that soybean miller was inoculated Trichoderma T115D fermentation and Phytophthora sojae in the same time was better than biocontrol effects that soybean miller was inoculated Trichoderma T115D fermentation in advance. When we used puring root inculation, different measure of inculation of Trichoderma T115D fermentation can alleviate Phytophthora root rot of soybean. Biocontrol effects that soybean miller was inoculated Trichoderma T115D fermentation of include spores (15 mL) and Phytophthora sojae (3 mL)in the same time was best, the Biocontrol effects value is 70.9%.