Study On Screening Of Good Fineness Germplasms And Expression Of Bast Fiber Development Relative Gene In Boehmeria Nivea(L.) Gaud

Ramie (Boehmeria nivea (L.) Gaud)is an important bast fiber crop, whose fiber which is one of the best bast fibers, is widely used in textile industry and plays an indispensable and irreplaceable role in the national economy. But now the fiber quality of ramie accessions are difficult to meet the high-textiles, small thin product requirements. Agronomic characters and bast fibre quality characters of totally 213 collected accessions of ramie were evaluated, and the genes expression related with fiber development were detected in different development period, between different genotypes by the semi-quantitative PCR. The main results were as follow:1. Yeild per rootstock and plant Height , diameter of stem, thickness of bark, rate of effective tiller, fibre rate of fresh bark existed a significant positive correlation relationships. There was a significant positive correlation between the raw ramie length and plant Height, diameter of stem, thickness of bark, rate of effective tiller, fibre rate of fresh bark, yeild per rootstock .It was a significant negative correlation between fibre fineness and plant Height, diameter of stem, thickness of bark, rate of effective tiller, fibre rate of fresh bark.2. There was significantly variance among agronomical characters and bast fibre quality characters. The Maximum coefficient of variation of yeild per rootstock was 47.27 %, the minimum is 0.08%, and that The Maximum coefficient of variation of fibre fineness was 29.56% and the minimum was 0.07%.3. 56 varieties were found to be high yield(≥110g). 38 varieties were identified as germplasm with good fineness(≥2200m/g). 5 varieties were identified as the accessions with excellently high yield (≥110g)and high fineness(≥2200m/g).4. The best optimal conditions amplifying fragments FB27 and CFE-1 at the same time could be screened: the best concentration of magnesiumions is 3.0 mmol L-1, annealing temperature is 55.6℃, 35 cycles.5. The relative expression of FB27 gene is 0.294 in the root, 0.845 in skin stem, 0.347 in leaves, 0.267 in shoot tip, 0.719 in stem bone. The general rule is that, bast> stem bone>leaf> root>shoot tip. FB27 expressed in various organs showed that FB27 gene expression is non-specific organizations, but the highest expression in the term of strong growth of stem, in particular, bast. The relative higher expression existed in high-fiber fineness germplasms, and the relative lower expression existed in low-fiber fineness germplasms. The relative gene expression of FB27 is that first-crop> third-crop > second-crop.6. CFE-1 gene was expressed in various organs, the general rule of the relative expression was that, bast> stem bone> shoot tip> leaf> root. The relatively higher expression existed in high-fiber fineness germplasms was significantly higher than those in low-fiber fineness germplasms. The relative gene expression of CFE-1 in Harvest periods was that first-crop> third -crop > second -crop.