The Analysis Of Genetic Diversity Of Three Species In Trifolium L. Determined By RAPD Markers

Clovers (Trifolium) are an important group of species of one genus in the world as famous cultivated forage grass as well as lawn grass. In general, clovers inhabit temperature regions of the world. Because of this, clovers cannot grow in Inner Mongolia Autonomous Region with drought and cold climate. But in some area in the north of Inner Mongolia Autonomous Region and Xinjiang Uygur Autonomous Region, for heavy snows in the winter, three species of clovers, white clover, red clover and T. lupinaster can survive in severe winter. In order to expand the clovers, three speices of clovers were used to detect the genetic diversity.20 materials belong to three species of clovers, which were collected from Inner Mongolia Autonomous Region and Xinjiang Uygur Autonomous Region, were analysed by Random Amplified Polymorphism DNA (RAPD). Based on analysis data, discuss the genetic diversity in and among species, and plants'blood relationship, through to special fragment screening appraisal, so that can obtain the special mark to lay the foundation for germplasm resource and breeding improved of clovers.20 clover materials were analyzed by RAPD markers to determine their genetic relationship. Of 100 random nucleotide primers screened, 7 showed steady banding patterns and produced 121 bands with 117 polymorphisms. Using un-weighted pair-group method with arithmetic means (Abbreviation UPGMA), constructs the phylogeny tree and acts according the phylogeny tree, according to UPGMA gathers cluster. The analysis result in species indicated that, the result of gathers cluster were more relative with the geography and the climate; it showed more different between cultivated variaties and wild materials; among the white clover, the material collected from Yakeshi had further distance from others. Another gather among species indicated that white clover and red clover were more similar compared to T. lupinaster. Obtain 3 special mark bands with good duplication and strong signal, OPB08-257 and OPC11-745 can take special mark bands to separate three clovers, and OPB08-300 may identify Haifa clover and other wild white clover.