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Effects Of Subacute Rumen Acidosis On Rumen Fermentation And Populations Of Lactate Metabolism Bacteria

Posted on:2009-03-31Degree:MasterType:Thesis
Country:ChinaCandidate:D ZhangFull Text:PDF
GTID:2143360245965881Subject:Prevention of Veterinary Medicine
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Six GuanZhong dairy goats in lactating period were used that had similar weights and steered with ruminal cannula. The trial was divided into four stages, the ratio of concentrate and roughage of four stages were 5:5, 6:4, 7:3 and 8:2 respectively. Ruminal pH was monitored in 24 hours uninterruptedly. According to the time that ruminal pH remained 5.5~5.8, determined whether SARA happened or not. The results of monitored ruminal pH showed that the goats were induced SARA in the level of 80% concentration. A study on rumen fermentation,blood-gas index,the populations of total bacteria and lactate-metabolizing bacteria (Strepococcus bovis, Lactobacillus, Selenomonas ruminantium and Megasphera elsdenii) using oligonucleotide probes to 16S rRNA,traditional cultivation method and Real-Time PCR was carried out under SARA situation. The results of this research as followed:1. Extend of increased butyrate was the most in three kinds of VFA. The increase of butyrate leaded to total VFA increased significantly (P<0.05); the concentration of lactate was lower than detection threshold in former three stages; it increased slightly under SARA condition.2. From the beginning to SARA happened, the concentration of total bacteria increased gradually, the population increased significantly under SARA situation especially (P<0.01).3. The populations of Lactobacillus and Strepococcus bovis increased following the percentage of concentrate increased. Obvious increase of Lactobacillus occurred until the level of 70% concentrate (P<0.05). Significant increase of Strepococcus bovis occurred until 80% concentrate (P<0.01). The population of Strepococcus bovis showed a slight increase by Real-Time PCR (P>0.05).4. The populations of Selenomonas ruminantium and Megasphera elsdenii increased significantly under SARA stage (P<0.01). There were no siginificant differences between the former there stages (P>0.05).5. When SARA happened, the pH of blood decreased (P>0.05), pCO2 of plasma increased , concentration of HCO3-decreased, BE of blood exhausted, AnGap's concentration increased(P<0.05); The concentrations of Na+, Ka+ and Cl- were influenced slightly with SARA.
Keywords/Search Tags:SARA, Rumen fermentation, Rumen bacteria, Lactate-production bacteria, Lactate-utilizing bacteria
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