| PART ONE Investigation of Nipah Virus (NiV) Natural Infection of equine in Yili, Xinjiang Uygur Autonomous Region. OBJECTIVE:To investigate the natural infection of Nipah Virus (NiV) of equine in Yili, Xinjiang Uygur Autonomous Region and explore if the nucleotide sequence in NiV N was homophylic with the overseas strains, detected from human and animals. METHODS: The N fragment of NiV was detected by one step real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR) in peripheral blood mononuclear cells (PBMCs) from 120 equines and in brain tissues (left frontal, parietal, temporal, occipital and midbrain) from 60 equines. And then the products of real-time RT-PCR from positive samples were cloned. Gene sequence and amino acid sequence were sequenced and analyzed before compared with those of the overseas strains, detected from human and animals. RESULTS: The positive rate of NiV N in PBMCs from 120 equines was 0% (0/120). The positive rate of NiV N in brain tissues from 60 equines was 0% (0/60). CONCLUSION: The investigation do not support that NiV infection exists in equine in Yili, Xinjiang Uygur Autonomous Region. PART TWO Investigation of NiV Natural Infection of dog in Yili, Xinjiang Uygur Autonomous Region OBJECTIVE:To investigate the epidemics of Nipah virus (NiV) of dog in Yili, Xinjiang Uygur Autonomous Region and explore if the nucleotide sequence in NiV N was homophylic with the overseas strains, detected from human and animals. METHODS: The N fragment of NiV was detected by one step real-time RT-PCR in PBMCs from 120 dogs. And then the products of real-time RT-PCR from positive samples were cloned. Gene sequence and amino acid sequence were analyzed and sequenced before compared with those of the overseas strains, detected from human and animals. RESULTS: The positive rate of NiV N in PBMCs from 120 dogs was 0% (0/120). CONCLUSION: The investigation do not support that NiV infection exists in dog in Yili, Xinjiang Uygur Autonomous Region.
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