Effects Of Dietary Energy Level And Source On The Expression Of ATP5A1, UCP2, Na~+-K~+-ATPase MRNA In Different Tissue And Orgains Of Gilts

Fifty-four Landrace×Yorkshire crossbred gilts,with initial age of 142±3d and initial body weight of 59±4.2 kg,were allotted to a3×2 factorial experiment to study the effects of dietary energy level and source on the physiological mechanisms of regulating the energy utilization and efficiency through the relation of weight and internal organ,gene expression of ATP5A 1,UCP-2 andNa+-K+-ATPase-alpha submit mRNA which related to energy metabolism and concentrations of serum T3 and T4. Three energy levels were 87.5%(L),100%(M),112.5%(H) NRC(1998) recommended energy requirement,respectively,and two energy source were starch(S) and fat(F, with equal proportion of pork fat and colza oil).Gilts were slaughtered on day 19 of the 2nd estrus cycle with slaughter age of 248±17d and slaughter body weight of 141±12.0 kg.The organs were collected and gave up the adipose.Detected the expression of gene ATP5A 1,UCP-2 andNa+-K+-ATPase-alpha submit mRNA in liver,back-adipose, jejunal mucous membrane and gastrocnemius.The results showed:1.The indexes of internal organs were decreased with the energy levels enhancing, but adipose tissue(including coelio-adipose,mesostenium and mesentery of large intestine) were increased with the energy levels enhancing(P＜0.05).The weight of spleen and lung were greater in L than in H(P＜0.01) and no significant difference in H and M and in M and in L(P＞0.05).There was no significant difference in others organ's weight(P＞0.05).2.ATP5A1 mRNA expression in liver was decreased with the energy level enhancing and the difference was significantly(P＜0.01) in H and L and no significant difference in H and M and in M and in L(P＞0.05).The expressions in the back-adipose and jejunal mucous membrane were higher in H than in L(P＜0.05) and no significant difference in H and M and in M and in L(P＞0.05).There was no significant difference in L and M and in M and H in the gastrocnemius(P＞0.05). ATP5A1 mRNA expression in liver,back- adipose,jejunal mucous membrane and gastrocnemius were lower in F than in S and the difference was significantly(P＜0.05) in liver and jejunal mucous membrane.There were interaction effectiveness of energy level and source on the back-adipose and jejunal mucous membrane(P＜0.01).3.UCP2 mRNA expression in back- adipose and gastrocnemius were lower in L than in H(P＜0.05) and there were no significant difference in H and M and in M and L (P＞0.05).The expression was higher in L than in H in the jejunal mucous membrane (P＜0.01) and no significant difference in L,M and H in liver(P＞0.05).UCP2 mRNA expression in the liver and gastrocnemius were higher in F than in S and the difference was significant(P＜0.05) in gastrocnemius.Expression in the back- adipose and jejunal mucous membrane were lower in F than in S but no significant difference(P＞0.05). There were interaction effectiveness of energy level and source on these organs (P＜0.01).4.The expression ofNa+,K+-ATPase alpha-subunit mRNA in liver,back-adipose and gastrocnemius were decreased with the increasing of dietary energy level and the difference was not significant(P＞0.05) in each energy level.The expression was higher in L than in H(P＜0.01) in jejunal mucous membrane.Na+,K+-ATPase alpha-subunit mRNA expression in the liver and jejunal mucous membrane were lower in F than in S and the difference was significantly(P＜0.01) in liver.There were interaction effectiveness of energy level and source on these organs(P＜0.01).5.There were significant positive relationship between the expression of ATP5A1 mRNA and UCP2 mRNA or Na+,K+-ATPase mRNA in liver(P＜0.05) and was negative relationship in back fat,jejunal mucous membrane and gastrocnemius,but no...