Study On Genetic Diversity And Protection Of Hippophae Gyantsensis And Hippophae Rhamnenoide Subsp. Yunnanensis

Hippophae gyantsensis and Hippophae rhamnenoide subsp,yunnanensis which are natural plant germplasm resources belong to Hippophae of Elaeagnaceae, play an important role in economic and ecological benefits.Hippophae gyantsensis and Hippophae rhamnenoide subsp,yunnanensis are both endemic species to the East Himalaya-Hengduan Mountains.For human activity in this area,the eco-environment becomes more and more fragiler which is harm to genetic diversity and genetic structure of Hippophae gyantsensis and Hippophae rhamnenoide subsp,yunnanensis, and there is not enough data to estimation the possibility of disappearance of genetic diversity.Classfying of protection category of the species have not done yet.In this paper,populations of Hippophae gyantsensis and Hippophae rhamnenoide subsp. yunnanensis in the distributing area are stdudied.We sequencing DNA of 109 individuals in 14 populations to analyse genetic diversity and genetic structure of the species.Risk of the genetic diversity lose of the species is studied based on the results.1.Study on genetic diversity and genetic differentiation of Hippophae gyantsensis and Hippophae rhamnenoide subsp,yunnanensisby seqencing gene TrnS-G and gene TrnL-F in Hippophae gyantsensis and Hippophae rhamnenoide subsp.Yunnanensis 11 haplotypes are occured.At the species level,high total diversity(Ht)are found,0.850 and 0.919 respectively for Hippophae gyantsensis and Hippophae rhamnenoide subsp,yunnanensis,and nucleotide diversity (π) are 0.00668 and 0.01159 respectively,the total gene diversity was 0.884. ARLEQUIN showed strong cpDNA genetic differentiation(Gst=0.694),64.42%of the total genetic diversity was partitioned among populations.Evidence for phylogeographical structure was detceted(Nst=0.725,significantly higher than Gst,P＜0.05).Older genetypes are infered from the minimum spaning tree which showed relatonship among the genetyoes.The result showed that there is no gene flow except haplotype H2 and H11.And we infere that there are several possible refugia.2.Risk for disperse of the genetic diversity of Hippophae gyantsensis and Hippophae rhamnenoide subsp,yunnanensisLow risk for genetic diversity of Hippophae gyantsensis and Hippophae rhamnenoide subsp,yunnanensis lose inferred from the study which showed high genetic diversity and strong differentiation in Hippophae gyantsensis and Hippophae rhamnenoide subsp.Yunnanensis.