Function Analysis Of The Genes Specifically Expressed In Gossypium Hirsutum During Fiber Development

Cotton is one of the most important economic crops in the world, and cotton fiber is the major natural fiber material for textile industry. Apart from the economic importance of fiber, developing cotton fiber is a perfect experimental model for studing the mechanism of cell elongation, wall development, cellulose biosynthesis in plants. Fibers are the seed hairs of cotton, and they originate from the epidermal cells of the ovular surface. Fiber development is composed of four distinct and overlapping stages. Isolation and analysis of genes expressed preferentially in cotton fiber will be helpful in further understanding the complicated process of fiber cell elongation and cellulose biosynthesis and ultimately does good to improve cotton fiber quality and yields.Our research are based on two genes GhFLA1 and GhAGP3. they were specifically expressed during cotton fiber development. Northern hybridization with specific probes and rt-pcr revealed that GhFLA1 abundantly expressed from 5DPA to 15DPA.GhAGP3 abundantly expressed on 15DPA ,and accumulated from 15DPA to 21DPA,but in the G. barbadense,it abundantly expressed untill 25DPA.A11 analysis results hinted GhFLAl may play vital roles during fiber rapid elongation and the primary cell wall development. But GhAGP3 might be involved in the secondary wall deposition.Furthermore we used the RNA interference approach to verify the precise functions during cotton fiber development of the two genes-GhFLA1 and GhAGP3.The silencing vectors of GhFLA1 and GhAGP3 were respectively transformed into upland cotton mediated by Agrobacterium. All RNAi constructs had obtained the transgenic plants. Some abnormal characteristic during the regermination may reveal that AGP3 associated in somatic embryo genesis. Cell expansion. and differentiation.