| Streptococcus suis is an important zoonotic pathogen causing mainly septicaemia, meningitis, endocarditis and endocarditis. In china, Streptococcus equi subsp.zooepidemicus had been identified as a major cause of contagious disease in pigs several decades ago. This situation has been changing from 1990, more and more other serotypes of Streptococcus suis have been isolated from diseased animals, serotype 2 has been considered as the most frequently isolated serotype and caused prevalence in some areas.Bacteria adhering to the tissular of host is the first step of colonization and developing infectious diseases. Sooner or later, it will develop bacteremia, sometimes septicemia, and finally meningitis. A recent study suggested that S. suis proteins have a role as adhesins to host cell surfaces, such as epithelial cells, phagocytes and brain microvascular endothelial cells.As one of the more common plasma proteins, serum albumin is bound to the surface of human group C and G streptococci, presumably in support of bacterial virulence. In vitro studies have shown that albumin-binding bacterial strains exhibit increased growth rates in the presence of human serum albumin compared to those which were grown in the absence the ligand—possibly benefiting from access to nutrients bound by the albumin.The differences of essential biology characteristic between Streptococcus suis type 2 and Streptococcus equi subsp. zooepidemicus were compared. The results showed that they are very different. The polyclonal antibodies of Streptococcus suis type 2 and Streptococcus equi subsp. zooepidemicus were prepared, and their titres were both 1:512. The bindings of serum albumin to Streptococcus suis type 2 and Streptococcus equi subsp. zooepidemicus were identified by enzyme-labeled immunosorbent assay. The results suggested that there are many agents can impact the bindings of serum albumin to Streptococcus suis type 2 and Streptococcus equi subsp. zooepidemicus, the bindings of serum albumin from different animals to Streptococcus suis type 2 and Streptococcus equi subsp. zooepidemicus are different, the adhesins of Streptococcus suis type 2 and Streptococcus equi subsp. zooepidemicus are both proteinaceous, and there are more adhesions binding to serum albumin on Streptococcus equi subsp. zooepidemicus surface than that on Streptococcus suis type 2 surface.The differences between Streptococcus suis type 2 and Streptococcus equi subsp. zooepidemicus can be used to distinguish them. Identifying the bindings of serum albumin to Streptococcus suis type 2 and Streptococcus equi subsp.zooepidemicus by enzyme-labeled immunosorbent assay is considered to be the point of innovation of this experiment. |
