QTL Basic Analysis And Physiological Character For Cold Tolerance At The Booting Period In Rice

In this study,using 107 individual plants of Hybrid F₂ from "maonuo/R527" and 108 individual plants of Backcross group,QTL and physiological indexes of cold tolerance at the booting stage in rice were analyzed,the results were as follows:1.In F_2:3 line group,examined cold tolerance of booting stage display continuous variation and approximately normal distribution,indicating cold tolerance was quantitative inherited.2.Under low temperature treatment,using the amendment of seed-setting rate in the cold tolerance identification may get reliable results.Similarly,chlorophyll and proline were also important indexes.3.Principal component and path analysis of physiological and morphological indexes indicated that the compositive analysis of physiological and morphological indexes was more effective in the research of cold tolerance in rice.4.In F_2:3 line group,a molecular linkage map of 47 SSR markers was constructed by internal mapping.Using mapmaker3.0,total 4 QTLs were detected on chromosome 1,3,6 and 7 respectively,named as qBS-1,qBS-3,qBS-6 and qBS-7 respectively.The values of LOD were 4.02,5.18,3.24 and 4.20 respectively,the variation of phenotype were 18.6%,20.3%,9.7%and 16.4%respectively.5.In Backcross group,a molecular linkage map of 51 SSR markers was constructed by using internal mapping,Under cold treatment,total 5 QTLs were detected on chromosome 1,3,6,7 and 11 respectively,named as qHBS-1,qHBS-3, qHBS-6,qHBS-7 and qHBS-11 respectively.The values of LOD were 3.04,5.70,2.50, 4.07,and 3.10 respectively,the variation of phenotype were 12.4%,24.2%,14.7%, 10.2%and 6.4%respectively.6.The results of backcross group was better than F_2:3 group.The genetic distance of selfing group was longer than baekcross group;RM473 primer on chromosome 1 was no self-chain in selfing group.In backcross group,a QTL of cold tolerance were found on the chromosome 11.